Protein Info for RR42_RS14675 in Cupriavidus basilensis FW507-4G11

Annotation: DNA topoisomerase IV subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 PF02518: HATPase_c" amino acids 36 to 177 (142 residues), 53 bits, see alignment E=8.6e-18 PF00204: DNA_gyraseB" amino acids 268 to 405 (138 residues), 114.3 bits, see alignment E=9e-37 PF01751: Toprim" amino acids 433 to 539 (107 residues), 42.7 bits, see alignment E=1.1e-14 PF00986: DNA_gyraseB_C" amino acids 589 to 653 (65 residues), 81.7 bits, see alignment E=6.8e-27

Best Hits

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 94% identity to cti:RALTA_A2160)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y4Q2 at UniProt or InterPro

Protein Sequence (663 amino acids)

>RR42_RS14675 DNA topoisomerase IV subunit B (Cupriavidus basilensis FW507-4G11)
MASKISQYSESSIRVLKGLEPVKQRPGMYTRTDNPLHIVQEVIDNASDEALGGHGSEIMV
TLHRDDSISVEDDGRGIPVGIHPEEQVPVVEIVFTRLHAGGKFDKGKGGAYAFSGGLHGV
GVSVTNALSTRLDVTVWRDSNCSTLTFSGGDVTVPLATRKLERGEKKSGTRVQVWPDAKY
FDSAAIPMAELQRLLRSKAVLLPGVKVTLLQEKNGERQTWQYEQGLKGYLVEALAQGSGA
ELVVPMFEGEHFADPDANPAEDGFAEGEGASWVVAWTEDGAPVRESYVNLIPTPAGGTHE
SGLREGLFQAVKSFIEMHALQPKGVKLMSEDVFARASFVLSAKVLDPQFQGQIKERLNSR
DAVKLVSTFSRPALELWLNHHVEYGKKLAELVIRQAQARTRAAQKVEKKKGSGVAVLPGK
LTDCESTDVTRNELFLVEGDSAGGSAKMGRDKEFQAILPLRGKVLNTWETERDRLFANNE
VHDIAVAIGVDPHGPNDEPDLSNLRYGKICILSDADVDGAHIQVLLLTLFFKHFPRLIDN
GNVCVARPPLYRVDAPARGKKPAQKLYALDDGELVAIQDKLMKDGIKEGGWQISRFKGLG
EMNAEQLWETTMNPDTRRLLPVALGHFDLPQTVGVMNMLMGKGEAAQRRTWLEERGNEVI
ADI