Protein Info for RR42_RS14645 in Cupriavidus basilensis FW507-4G11

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 TIGR01228: urocanate hydratase" amino acids 11 to 554 (544 residues), 1015.5 bits, see alignment E=0 PF17391: Urocanase_N" amino acids 12 to 138 (127 residues), 211.4 bits, see alignment E=4.5e-67 PF01175: Urocanase" amino acids 141 to 349 (209 residues), 291.9 bits, see alignment E=4.3e-91 PF17392: Urocanase_C" amino acids 352 to 546 (195 residues), 306.5 bits, see alignment E=9.7e-96

Best Hits

Swiss-Prot: 91% identical to HUTU_PSEA7: Urocanate hydratase (hutU) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 91% identity to pap:PSPA7_5833)

MetaCyc: 91% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.49

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YHZ5 at UniProt or InterPro

Protein Sequence (561 amino acids)

>RR42_RS14645 urocanate hydratase (Cupriavidus basilensis FW507-4G11)
MSNTDRFRDVEIRAPRGNQLNAKSWQTEAPLRMLMNNLDPEVAENPKELVVYGGIGRAAR
NWACYDKIVETLKTLNDDETLLVQSGKPVGVFKTHGNAPRVLIANSNLVPHWATWEHFNE
LDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYNGNLKGRWVLTAGLGGMGG
AQPLAATLAGACSLNIECQQASIDFRLRTRYVDEQATDLDDALARIARYTAEGRAVSIAL
CANAAEVLPELVRRGVRPDMVTDQTSAHDPLNGYLPVGWTWGQYRDRAQTEPARVVKAAK
QSMALHVQAMLDFQKLGVPTFDYGNNIRQMAKEEGVANAFDFPGFVPAYIRPLFCRGVGP
FRWAALSGDPQDIYKTDAKVKELIPDDAHLHRWLDMARERISFQGLPARICWVGLGLRAK
LGLAFNEMVRSGELSAPVVIGRDHLDSGSVASPNRETESMQDGSDAVSDWPLLNALLNTA
SGATWVSLHHGGGVGMGFSQHSGVVIVCDGTDEAAARIARVLHNDPATGVMRHADAGYDI
AIDCAREQGLNLPMLNSKKEA