Protein Info for RR42_RS14385 in Cupriavidus basilensis FW507-4G11

Annotation: 3-isopropylmalate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00169: 3-isopropylmalate dehydrogenase
  • amino acids 2 to 349 (348 residues), 485.9 bits, E=3.2e-150
• PF00180: Iso_dh
  • amino acids 2 to 351 (350 residues), 446.8 bits, E=3.1e-138

Best Hits

Swiss-Prot: 96% identical to LEU3_CUPMC: 3-isopropylmalate dehydrogenase (leuB) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 95% identity to reu:Reut_A2311)

MetaCyc: 57% identical to beta-isopropylmalate dehydrogenase subunit (Leptospira interrogans serovar Lai str. 56601)
3-isopropylmalate dehydrogenase. [EC: 1.1.1.85]; 1.1.1.- [EC: 1.1.1.85]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (6/8 steps found)
• butanol and isobutanol biosynthesis (engineered) (4/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Sequence Analysis Tools

See A0A0C4Y4K6 at UniProt or InterPro

Protein Sequence (355 amino acids)

>RR42_RS14385 3-isopropylmalate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MKIAVLPGDGIGPEIIAEAVKVLNALDEKFEMETAPVGGAGYEASGHPLPDATLKLAQEA
DAILFGAVGDWKYDKLDRPLRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA
GLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK
RGKKLCSVDKANVLETFQFWKDVVIDVHKEYPEVELSHMYVDNAAMQLIKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSA
AMMLRFSLNRAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTREMGAAVLAAL