Protein Info for RR42_RS14190 in Cupriavidus basilensis FW507-4G11
Annotation: 2-hydroxyacid dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 44% identical to GHRB_CITK8: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
KEGG orthology group: None (inferred from 87% identity to cti:RALTA_A2089)MetaCyc: 49% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]
Predicted SEED Role
"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)
MetaCyc Pathways
- formaldehyde assimilation I (serine pathway) (10/13 steps found)
- glucose degradation (oxidative) (4/5 steps found)
- ketogluconate metabolism (6/8 steps found)
- L-serine biosynthesis II (3/4 steps found)
- L-arabinose degradation IV (5/8 steps found)
- D-xylose degradation IV (4/7 steps found)
- superpathway of pentose and pentitol degradation (13/42 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glutathione metabolism
- Glycine, serine and threonine metabolism
- Glyoxylate and dicarboxylate metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose phosphate pathway
- Polyketide sugar unit biosynthesis
- Pyruvate metabolism
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.43, 1.1.1.79, 1.1.1.81
Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.43 or 1.1.1.79 or 1.1.1.81
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4YBM3 at UniProt or InterPro
Protein Sequence (332 amino acids)
>RR42_RS14190 2-hydroxyacid dehydrogenase (Cupriavidus basilensis FW507-4G11) MTKPSVLVTRAVFPEVIDRLAQYFDVDDNQPDVALDGAALKARLAGKAGVLANAADRIDA GLVAGLPALRAVCNMAVGYNNLDVPALTAAGIVATNTPDVLTETTADFGWALLMATARRV TESEHWLRAGKWERWSYDMFLGMDLYRSTLGILGMGRIGQALARRAAGFGMSVLYHNRSR LPEDTERVLNARYVSKAELLAQSDHLLLVLPYSAQSHHSIGAAELAQMKPSATLVNLARG GVVDDAALARALREGRIFAAGLDVFEAEPQVHPDLLTVPNVVLTPHIASASEKTRRAMAD LAADNLIAALGVGPQAGQPPSVINPEVMARRR