Protein Info for RR42_RS13510 in Cupriavidus basilensis FW507-4G11
Annotation: carbamoyl phosphate synthase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 69% identical to CARB_ECO57: Carbamoyl-phosphate synthase large chain (carB) from Escherichia coli O157:H7
KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 68% identity to abb:ABBFA_000786)MetaCyc: 69% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]
Predicted SEED Role
"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- superpathway of arginine and polyamine biosynthesis (15/17 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- UMP biosynthesis I (6/6 steps found)
- UMP biosynthesis II (6/6 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- L-citrulline degradation (3/3 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- UMP biosynthesis III (5/6 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
- allantoin degradation IV (anaerobic) (4/9 steps found)
- guadinomine B biosynthesis (2/13 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.5
Use Curated BLAST to search for 6.3.5.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4Y486 at UniProt or InterPro
Protein Sequence (1082 amino acids)
>RR42_RS13510 carbamoyl phosphate synthase large subunit (Cupriavidus basilensis FW507-4G11) MPKRTDIKSILIIGAGPIIIGQACEFDYSGAQACKALREEGFKVVLVNSNPATIMTDPNT ADVTYIEPITWEVVERIIAKERPDAILPTMGGQTALNCALDLHRHGVLAKYNVELIGASP EAIDKAEDRQKFKEAMTKIGLGSAKSGIAHSMEEALAVQTQIAKETATGGYPIVIRPSFT LGGSGGGIAYNREEFEEICKRGLDLSPTRELLIEESLLGWKEYEMEVVRDKKDNCIIICS IENLDPMGIHTGDSITVAPAQTLTDKEYQILRNASLAVLREIGVDTGGSNVQFSINPADG RMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNEITGGATPASFEPSID YVVTKVPRFAFEKFPQADSHLTTQMKSVGEVMAMGRTFQESFQKALRGLEVGVDGLDDKS TDRDEIVEEIGEAGPDRIWYVGDAFRIGMSLEEVHAETAIDPWFLAQIEDIVKTETLVKA RKLDSLSAAELRHLKQKGFSDRRLAKLMGAEPAAVRVARHAAGVRPVYKRVDTCAAEFAT NTAYMYGTYEAEHGECEADPTANRKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYET IMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIVDREKPVGVIVQYGGQTPLKLALDLEA NGVPIIGTSPDMIDAAEDRERFQKLLHELGLRQPPNRTARAEDEALRLATEIGYPLVVRP SYVLGGRAMEIVHEPRDLERYMREAVKVSHDSPVLLDRFLNDAIECDVDALCDGQRVFIG GVMEHIEQAGVHSGDSACSLPPYSLAQATVDELKRQTAAMAKALNVIGLMNVQFAIQQVN GEDIVYVLEVNPRASRTVPYVSKATGLSLAKIAARCMAGQTLDSQGVFDEVVPPYFSVKE AVFPFNKFPGVDPVLGPEMRSTGEVMGVGKTFGEALFKSQLAAGSRLPEKGTVLLTVKDS DKPHAVGVARMLHDMGYPIVATRGTASAIEAAGIPVRVVNKVKDGRPHIVDMLKNGELAL VFTTVDETRTAIADSRSIRISALASRVPYYTTIAGARAAVEGLKHMQSLEVYDLQSLHAS LA