Protein Info for RR42_RS13290 in Cupriavidus basilensis FW507-4G11

Annotation: ribonucleoside-diphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 839 transmembrane" amino acids 367 to 385 (19 residues), see Phobius details PF00317: Ribonuc_red_lgN" amino acids 16 to 82 (67 residues), 33.7 bits, see alignment E=3e-12 TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent" amino acids 28 to 587 (560 residues), 596.1 bits, see alignment E=4e-183 PF02867: Ribonuc_red_lgC" amino acids 89 to 580 (492 residues), 415 bits, see alignment E=4.7e-128

Best Hits

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 82% identity to reh:H16_A2390)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YAW7 at UniProt or InterPro

Protein Sequence (839 amino acids)

>RR42_RS13290 ribonucleoside-diphosphate reductase (Cupriavidus basilensis FW507-4G11)
MNAKDQMLRATLPPQDISTEVLLEKYAKGEEATAADVRQRVARALAEAEPEAQRAQWAER
FLWAQEQGFVPAGRINSAAGTRIEATLINCFVQPVGDSVSEVRDGKPSIYTAVAQAAETM
RRGGGVGYDFSAIRPAGALVRGTHSRASGPVSFMKVFDASCATVESAGSRRGAQMGVLRC
DHPDIETFIHAKDRGELTNFNISIGVSDDFMRAVEADGEVELVHEAEPAAETIAAGAYRR
DDGQWAYRRVPARQLWDQVMRATYDHAEPGILFLSHINDDNNLYYCERIEATNPCAEQPL
PPYGCCDLGSIDLTRFVRQPFGADSRFDFEAFAEVVRLAVRMLDNVLDITYWPLPEQQAE
AHAKRRIGLGFLGLGSALVMLGIRYDAEAGRALAARIAQTLRDTAYLASAGLAREKGAFP
LFKAEAYLASGFARRLPGAVREAIGRDGIRNSHLLSIAPTGTITLAFADNASNGIEPAFS
WTYNRKKRMADDSYKVFEVADHAWRLYRHLGGDMAQLPDSFVTALQMSALDHMKMLEAVQ
PYIDTSISKTVNVPEDYPYEAFRNLYLEAWRAGLKGLATYRPNSVLGAVLSVTPPPAGAQ
AAPAMPAVTDDPLRKPFASRPVGDLEGVTSKVEYMTYEGHKTVYLTVNFMRVAGQLDGKP
VAVERPVEFFMPAGQRNDGQQWITSTMRLLSMVARSGGSIAKALADMRNVVWDKGTVRYG
TLTRQDGTEVPRFHDSEVAAIGYALQRILIKRGFLDAEGIQVPVAALAARLAGLEGLEGP
SGGAVDRGGAAPAAGGAGMPAAGMGLRSGKPCPECGAHALHKVDGCAKCANCGYVGECG