Protein Info for RR42_RS13060 in Cupriavidus basilensis FW507-4G11

Annotation: 23S rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00186: RNA methyltransferase, TrmH family, group 3
  • amino acids 4 to 243 (240 residues), 247.9 bits, E=4.8e-78
• PF08032: SpoU_sub_bind
  • amino acids 6 to 77 (72 residues), 60 bits, E=2.3e-20
• PF00588: SpoU_methylase
  • amino acids 97 to 237 (141 residues), 153.4 bits, E=4.4e-49

Best Hits

Swiss-Prot: 82% identical to RLMB_RALSO: 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03218, RNA methyltransferase, TrmH family [EC: 2.1.1.-] (inferred from 94% identity to cti:RALTA_A1893)

Predicted SEED Role

"23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See A0A0C4Y419 at UniProt or InterPro

Protein Sequence (247 amino acids)

>RR42_RS13060 23S rRNA methyltransferase (Cupriavidus basilensis FW507-4G11)
MAKQKLLIGFHAVTARLRQDAAGVTDVYVESARRDRRMQDFIRLAESLKVRLHPVDAERL
RGMAGTDRHQGVVARAEDVALALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADGA
GAHAVIAPKDRSVGLNTTVAKVASGAAETVPYITVTNLARTLRELQERGIWVIGTDDGTE
KTLYDVDFKGPVALVMGAEGEGMRRLTRETCDELVSIPMAGGVESLNVSVASGVCLYEAV
RQRRLKR