Protein Info for RR42_RS12830 in Cupriavidus basilensis FW507-4G11

Annotation: multidrug resistance protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 transmembrane" amino acids 30 to 49 (20 residues), see Phobius details amino acids 73 to 92 (20 residues), see Phobius details amino acids 100 to 121 (22 residues), see Phobius details amino acids 127 to 149 (23 residues), see Phobius details amino acids 161 to 182 (22 residues), see Phobius details amino acids 188 to 210 (23 residues), see Phobius details amino acids 222 to 241 (20 residues), see Phobius details amino acids 253 to 272 (20 residues), see Phobius details amino acids 293 to 315 (23 residues), see Phobius details amino acids 327 to 345 (19 residues), see Phobius details amino acids 356 to 375 (20 residues), see Phobius details amino acids 385 to 407 (23 residues), see Phobius details amino acids 499 to 518 (20 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 36 to 516 (481 residues), 510.5 bits, see alignment E=2.4e-157 PF07690: MFS_1" amino acids 40 to 433 (394 residues), 169.1 bits, see alignment E=1.3e-53 PF06609: TRI12" amino acids 42 to 312 (271 residues), 33.2 bits, see alignment E=2.2e-12

Best Hits

Swiss-Prot: 50% identical to FARB_NEIGO: Fatty acid resistance protein FarB (farB) from Neisseria gonorrhoeae

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 88% identity to reh:H16_A2298)

MetaCyc: 48% identical to tripartite efflux pump membrane subunit EmrY (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YCX4 at UniProt or InterPro

Protein Sequence (534 amino acids)

>RR42_RS12830 multidrug resistance protein B (Cupriavidus basilensis FW507-4G11)
MATSLPARDGASPGPAAPAARRAAPVPLSGAKLIVGTIALSLATFMNVLDSSIANVSIPA
ISGDLGVAPNQGTWVITSFAVANAISVPLTGWMTTRFGAVRLFVTSILLFVISSWLCGVA
PNLEVLLAARVLQGAVAGPMIPLSQSLLLSSYPPQKSTMALALWGMTTLVAPIMGPLLGG
WISDNMTWPWIFYINIPVGLGAAYATWAIYRDRETPIKILPIDRIGLALLVIWVGSMQLM
LDKGKELDWFHSTSIVVLTVVAIVGFLFFLIWESYEKHPIVDITLFKGRNFSAGVVAISV
AYGLFFGNLVILPLWLQTIVGYTATDAGIVMAPVGIFAILLSPLIGKNLPKMDARWVATL
AFVTFGVVSLMRSGFTLQVDTRTLMIPTLIQGAAMAMFFIPLTSIILSGQPPERIPSASG
VSNFVRITFGAIGASISTTIWENRTALHHAQLVEQVNPYNPTYIDQLNHLMQLGMSQAQA
NGQIERNISQQAAMLGANDIFWISGVLFFVLIVFVWLTKPKKGGDLDASAAGAH