Protein Info for RR42_RS12175 in Cupriavidus basilensis FW507-4G11

Annotation: malate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 8 to 69 (62 residues), 71.8 bits, E=4e-24
• TIGR01344: malate synthase A
  • amino acids 22 to 528 (507 residues), 736.1 bits, E=7.8e-226
• PF01274: MS_TIM-barrel
  • amino acids 160 to 404 (245 residues), 350.7 bits, E=4.9e-109
• PF20659: MS_C
  • amino acids 411 to 528 (118 residues), 133 bits, E=1.1e-42

Best Hits

Swiss-Prot: 57% identical to MASY_MYXXD: Malate synthase (mls) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 92% identity to reh:H16_A2217)

MetaCyc: 50% identical to malate synthase (Saccharomyces cerevisiae)
Malate synthase. [EC: 2.3.3.9]; 3-ethylmalate synthase. [EC: 2.3.3.9, 2.3.3.7]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• L-arabinose degradation IV (5/8 steps found)
• chitin deacetylation (2/4 steps found)
• D-xylose degradation IV (4/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (4/8 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)
• superpathway of pentose and pentitol degradation (13/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.7 or 2.3.3.9

Sequence Analysis Tools

See A0A0C4YGN2 at UniProt or InterPro

Protein Sequence (528 amino acids)

>RR42_RS12175 malate synthase (Cupriavidus basilensis FW507-4G11)
MAITLPAGMKITGEILPAYEDILTPAALALVDKLHRAFEPRRQELLAARVERTKRLDAGE
LPDFLPQTKSVREGDWKVAPVPPALHCRRVEITGPVEAKMVINAFNSGADSYMTDFEDSN
TPNWHNQLQGQVNLKAAVRRTLTLDSNGKHYKLNGKIATLQVRPRGWHLDEKHVTIDGQR
VSGGIFDFALFLFHNAKEQIARGAGPFFYLPKMESHLEARLWNDVFVMAQNEIGLPQGTV
KATVLIETILAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKVDKNFCLADRAKVTM
TAPFMRSYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMQGIINDKKRDATDGYD
GGWVAHPGLVEPAMKEFVAVLGDKPNQFDKQRDDVNVAAKDLLDFQPETPITEAGLRMNI
NVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGRKVTAEMVRA
LIPEELAKVKAFVGGDTTTYDRAGEIFEQMSTSEDFAEFLTLPLYEEI