Protein Info for RR42_RS11485 in Cupriavidus basilensis FW507-4G11

Annotation: dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 20 to 550 (531 residues), 777.7 bits, see alignment E=2.9e-238 PF13360: PQQ_2" amino acids 68 to 225 (158 residues), 57.3 bits, see alignment E=2.9e-19 amino acids 464 to 533 (70 residues), 34 bits, see alignment E=3.7e-12 PF01011: PQQ" amino acids 89 to 115 (27 residues), 23 bits, see alignment (E = 7.8e-09) amino acids 497 to 532 (36 residues), 35.7 bits, see alignment 7e-13 PF13570: PQQ_3" amino acids 108 to 156 (49 residues), 18 bits, see alignment 4.9e-07

Best Hits

Swiss-Prot: 54% identical to EXAA_PSEAE: Quinoprotein alcohol dehydrogenase (cytochrome c) (exaA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 93% identity to reu:Reut_A1784)

MetaCyc: 54% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YGC2 at UniProt or InterPro

Protein Sequence (580 amino acids)

>RR42_RS11485 dehydrogenase (Cupriavidus basilensis FW507-4G11)
MRTLRVMMVLAATTASLVAQAAVTDAMIENDAKSPGDVLSWGMGPQGQRFSTLNRINTKN
ISQLVPAWSFSFGGEKQRGQEAQPLIHDGKMFVTASYSRIYALDLKTGTKLWKYEHRLPE
GIMPCCDVVNRGAALYDNLVIFGTLDAQLVALDQKTGKVVWKEKIEDYAAGYSYTAAPLI
VKGMVLTGISGGEFGVVGRVEARDAKTGQMVWSRPVVEGHMGYKYDKDGNKTENGVTGTQ
NASWPGETWKTGGAATWLGGTYDPQTGLAYFGTGNPGPWNSHMRKGDNLYSASTVALDPA
TGKIVWHYQNTPNDGWDFDGVNEFVTFDQDGKRLGGKADRNGFFYVNDATSGKLVNAFPF
VKKITWASSIDLKTGRPNMIAESRPGDPAAGGDPKKGQSVFAAPGFLGGKNQQPMAYSPQ
TGLFYVPANEWGMDIWNEPVGYKKGAAFLGAGFTIHPLNEDYIGSLRAINPKTGKIEWEV
KNYAPLWGGVMTTAGGLVFWGTPEGYLKAADAKTGKELWKFQTGSGVVAPPVTWEEGGEQ
YVAVVSGWGGAVPLWGGEVAKRVNFLEQGGSVWVFKLHKS