Protein Info for RR42_RS08825 in Cupriavidus basilensis FW507-4G11

Annotation: nitrate/nitrite transporter NarK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 28 to 49 (22 residues), see Phobius details amino acids 65 to 86 (22 residues), see Phobius details amino acids 95 to 114 (20 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 160 to 188 (29 residues), see Phobius details amino acids 209 to 228 (20 residues), see Phobius details amino acids 252 to 274 (23 residues), see Phobius details amino acids 286 to 304 (19 residues), see Phobius details amino acids 314 to 333 (20 residues), see Phobius details amino acids 345 to 371 (27 residues), see Phobius details amino acids 399 to 420 (22 residues), see Phobius details amino acids 432 to 452 (21 residues), see Phobius details TIGR00886: nitrite transporter" amino acids 27 to 413 (387 residues), 349.2 bits, see alignment E=1.5e-108 PF07690: MFS_1" amino acids 32 to 368 (337 residues), 56.6 bits, see alignment E=1.1e-19

Best Hits

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 87% identity to reu:Reut_B5001)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y870 at UniProt or InterPro

Protein Sequence (461 amino acids)

>RR42_RS08825 nitrate/nitrite transporter NarK (Cupriavidus basilensis FW507-4G11)
MSSSVLTRWEPESAAFWRTHGERIAYRNLWISILALMLAFAIWMLWSVVAVNLDRAGFRF
SKNQLFWLTALPALSGATLRIFYAFLVPIFGGRRFTAISTATLLVPALGMGFALRDPATS
YPTLLVLALLCGFGGANFSSSMSNISFFFPKAKKGLATGLNAGIGNLGVSVVQFLTPLVV
SVGLFGAFGGEPQAYQAGEAGRSLWLQNAGFIWVPFILAATLAAWFGMNDIADARASFAD
QAVIFKRKHNWLMCWLYVGTFGSFIGFSAGFALLTKSQFPGVNPTAYAFLGPLMGALMRP
VGGWVSDKLGGARVTLWSFVAMIAAVFGVLATLPHDGAGGSFQGFLAAFVVLFALTGVGN
GSTFRMIPVIFLTERQRAARGKDAAAQKQAQLDAGKESAAVLGFSGAIGAYGGFFIPKSF
GTSLELTGSADAALYCFVVFYVSCVLVTWWYYARKNAPMPC