Protein Info for RR42_RS08820 in Cupriavidus basilensis FW507-4G11

Annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1246 transmembrane" amino acids 204 to 206 (3 residues), see Phobius details TIGR01580: nitrate reductase, alpha subunit" amino acids 2 to 1232 (1231 residues), 2129.3 bits, see alignment E=0 PF14710: Nitr_red_alph_N" amino acids 3 to 40 (38 residues), 60.8 bits, see alignment (E = 1.9e-20) PF00384: Molybdopterin" amino acids 108 to 828 (721 residues), 223.1 bits, see alignment E=8.3e-70 PF01568: Molydop_binding" amino acids 1084 to 1199 (116 residues), 74.1 bits, see alignment E=1.4e-24

Best Hits

Swiss-Prot: 66% identical to NARG_ECOLI: Respiratory nitrate reductase 1 alpha chain (narG) from Escherichia coli (strain K12)

KEGG orthology group: K00370, nitrate reductase 1, alpha subunit [EC: 1.7.99.4] (inferred from 67% identity to aaa:Acav_0630)

MetaCyc: 68% identical to respiratory nitrate reductase alpha subunit (Stutzerimonas stutzeri)
RXN-11236 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase alpha chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YAA4 at UniProt or InterPro

Protein Sequence (1246 amino acids)

>RR42_RS08820 nitrate reductase (Cupriavidus basilensis FW507-4G11)
MSHFLDRLKFMSRVKSTFAGGHGAVVDEDRRWENGYRSRWQHDKIVRSTHGVNCTGSCSW
KVYVKNGLITWETQQTDYPRTRPDLPNHEPRGCPRGASYSWYVYSAQRVKYPMIRGRLME
MWREARKTMDPVAAWASISQDPVKANRYKSVRGLGGFVRADWDTATEMIAAANAFTIKRF
GPDRVVGFSPIPAMSMVSYAAGARYLSLLGGVCLSFYDWYCDLPPASPQVWGEQTDVPES
ADWYNSTYLMVWGSNVPQTRTPDAHFYTEVRYKGTKTVAVSSDFGEMVKFGDIWLTPTQG
TDAALAMAMGHVVLKEFHLNARSAYFRDYIRQYTDMPMLVLLRDNGGTLVPDHFLRASHL
AGARGEANNPEWKTLVIDSASGDFAAPNGSIGFRWGEAAHNDGEKVGRWNLEMKDGGSGH
ALDPLLSLMAAEHEVAEVGFPYFGAEHDALLKRRVPARRITLADGSSALVATVYDLQMAN
YGVDQGLGGDNVATSFDDDVPYTPAWQEKHTTVPRHLVIQVAREFADNADRTQGKSMVIV
GAGLNHWYHNDMIYRGIINLLMMCGCVGKSGGGWAHYVGQEKLRPQFGWAPLAFGLDWSR
PPRQMNGTSFFYSHTSQWRHEKLAVDEILAPTADKAKYENLSLLDLNARSERMGWLPSAP
QLSSNPLDVADAAAAAGKDPVAYTVEQLKSGGLQFACDDPDNPENFPRNMFVWRSNILGS
SGKGHEYFLKYLLGTQNAVFGDEADAITPSEVKVRPAAEGKLDLLTVLDFRMSTTCLYGD
IVLPTATWYEKDDLNTSDMHPFIHPLSEAVQPLWESRTDWEIYKAIARKFSEIGGAHLGT
RKDLVCSPLLHDTPGELAQPFEPKDWKRGECELIPGKTAPSMTVVERNYADIYKKFTSIG
PLLDKHGNGGKGINWDTRHEVKEIGALSKTVDEPGVSQGRPRLDTAIDAAEMILTFAPET
NGHVAVKAWEALSAITGRDHAHLAAGREHDKIRFRDVQVQPRKIISAPTWSGLESEEVSY
NAGYTNVHELIPWRTLTGRQQFYQDHRWMLDFGEGNCVYKPAIDTKTIAPMLGAKPNGNT
ELVLNWITPHQKWGIHSTYSDNLRMLTLSRGGPHVWISETEAKASGIRDNDWVEVFNVNG
TLTARVVVSQRVPKGMCLMYHAQEKIVNVPGAETSGMRGGIHNSVTRTVTKPTHMIGGYA
QLAYGFNYYGTVGSNRDEFVILRKMNKVDWLEGPLVEKQAKEGAEQ