Protein Info for RR42_RS07465 in Cupriavidus basilensis FW507-4G11

Annotation: dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 6 to 342 (337 residues), 417.2 bits, E=2.2e-129
• PF01180: DHO_dh
  • amino acids 51 to 341 (291 residues), 264 bits, E=8.2e-83

Best Hits

Swiss-Prot: 91% identical to PYRD_CUPNH: Dihydroorotate dehydrogenase (quinone) (pyrD) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 90% identity to reu:Reut_A1317)

MetaCyc: 54% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

See A0A0C4Y9G7 at UniProt or InterPro

Protein Sequence (344 amino acids)

>RR42_RS07465 dihydroorotate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MLNALYPLFRPALFSMDAEDAHHFTLNNLLRAKRMGLAGCIGNTIADDPRTVMGVRFPNP
VGLAAGLDKDGAYIDGLAAFGFGFIEVGTVTPRPQAGNPRPRMFRLPQADALINRMGFNN
GGVDAFIANVNASRWKAEGGVLGLNIGKNADTPIERAVDDYLHCLERVYPHASYVTVNIS
SPNTKNLRQLQGASELDHLLCTLKDAQQRLADKYKRYVPLALKIAPDLDADQIGNIGDAL
VRHRIDGVIATNTTIARDAVRGLPHGEEAGGLSGRPVFESSTRVVKSLRAVVGDAVPIVG
VGGIFSGADAQAKIAAGAQLVQVYSGLIYRGPVLVKECAAALRA