Protein Info for RR42_RS07345 in Cupriavidus basilensis FW507-4G11

Annotation: methenyltetrahydrofolate cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 120 (115 residues), 149.3 bits, E=5.3e-48
• PF02882: THF_DHG_CYH_C
  • amino acids 123 to 281 (159 residues), 231.2 bits, E=3.9e-73

Best Hits

Swiss-Prot: 93% identical to FOLD_CUPNH: Bifunctional protein FolD (folD) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 93% identity to reu:Reut_A1299)

MetaCyc: 61% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• folate transformations II (plants) (10/11 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• folate transformations I (9/13 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See A0A0C4Y9F2 at UniProt or InterPro

Protein Sequence (283 amino acids)

>RR42_RS07345 methenyltetrahydrofolate cyclohydrolase (Cupriavidus basilensis FW507-4G11)
MPAQLIDGNALAKQLRTEAAQRAARLTERGHRPGLAVVLVGEDQASQVYVRNKVKACDDN
GFHSSLDRYPADLSEADLLARIDALNRDPHIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
GFHVANAGALMTGAPLFRPCTPYGCMKMLESVDFPLRGARAVVVGASNIVGKPMAMLLLQ
AGATVTICNSKTRDLGAHTRDADVIVAAVGRRNIITADMVKPGAAVIDVGMNRDDNGKLC
GDVDFNGVKEVAGYITPVPGGVGPMTITMLLINTLEAAERAAG