Protein Info for RR42_RS06130 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): Gluconokinase (EC 2.7.1.12)
Rationale: Specifically important for utilizing D-Gluconic Acid sodium salt. Automated validation from mutant phenotype: the predicted function (GLUCONOKIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: gluconate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 169 TIGR01313: carbohydrate kinase, thermoresistant glucokinase family" amino acids 3 to 153 (151 residues), 164 bits, see alignment E=1.3e-52 PF01202: SKI" amino acids 10 to 137 (128 residues), 38.5 bits, see alignment E=6.4e-14

Best Hits

Swiss-Prot: 44% identical to GNTK_ECOLI: Thermoresistant gluconokinase (gntK) from Escherichia coli (strain K12)

KEGG orthology group: K00851, gluconokinase [EC: 2.7.1.12] (inferred from 81% identity to reu:Reut_A1082)

MetaCyc: 44% identical to D-gluconate kinase, thermostable (Escherichia coli K-12 substr. MG1655)
Gluconokinase. [EC: 2.7.1.12]

Predicted SEED Role

"Gluconokinase (EC 2.7.1.12)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway (EC 2.7.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y113 at UniProt or InterPro

Protein Sequence (169 amino acids)

>RR42_RS06130 Gluconokinase (EC 2.7.1.12) (Cupriavidus basilensis FW507-4G11)
MIYILMGVSGSGKTTVGQLLAKTLQCGFHDADEFHSAANKAKMHNGIPLTDDDRWPWLAA
MRAAIDAARAEGRTHVFTCSALRQTYRDRLTPADGGVTFVYMKGDAAIIGGRLAERSEHF
FNPALLQSQFETLEEPREALVLDIRQTPEALVQCILAGVQAQKGVAIAR