Protein Info for RR42_RS06090 in Cupriavidus basilensis FW507-4G11

Annotation: single-stranded DNA exonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 transmembrane" amino acids 188 to 201 (14 residues), see Phobius details TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 23 to 563 (541 residues), 480.5 bits, see alignment E=2.7e-148 PF01368: DHH" amino acids 74 to 230 (157 residues), 87.4 bits, see alignment E=1.5e-28 PF02272: DHHA1" amino acids 354 to 449 (96 residues), 65.9 bits, see alignment E=5.8e-22 PF17768: RecJ_OB" amino acids 467 to 563 (97 residues), 66.1 bits, see alignment E=4.1e-22

Best Hits

Swiss-Prot: 47% identical to RECJ_HAEIN: Single-stranded-DNA-specific exonuclease RecJ (recJ) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 88% identity to reu:Reut_A1074)

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y6N5 at UniProt or InterPro

Protein Sequence (565 amino acids)

>RR42_RS06090 single-stranded DNA exonuclease (Cupriavidus basilensis FW507-4G11)
MTKIAVRSFSTEHAGKLAGFGLHPTLARILSARGVREPAELATELPGLAPPAAMKGIDHA
ATYLADAIAAGRRLLIVADYDCDGATACAVGVRGLRMLGARIDYIVPNRFEYGYGLTPEI
VALAARQQPDVIVTVDNGIASVDGVAAANALGIDVVVTDHHLPGDQLPDAAVIVNPNQPG
CEFPSKNLAGVGVMFYVLLALRAELRRRGVFDAQTQPRLDTLLDLVALGTVADVVKLDSN
NRILVAQGLKRMRAGRMHAGVAALFRAAGREATRANTFDLGFGVGPRLNAAGRLADMSLG
IECLLTDDANRAWEIAQELDGMNRERRDIEAGMQQEALQILEGPLGALDPAARFTVSVFN
DTWHQGVIGIVASRLKEKYHRPTITFAPGDEDTIKGSGRSIPGFHLRDALDLVSKRHPGL
LKKFGGHAMAAGLTLGADSFGQFMEAFEAVGREWLSDEQLARVIETDGDIEDACFSPEFV
SLLEQQVWGQGFPAPTFCGEFDVLRQSVLKGKHLKLQLGRGSQRFDAIWFNHADSLGASA
LVAYRLDNNTFNGITRVQMVIEHAQ