Protein Info for RR42_RS05890 in Cupriavidus basilensis FW507-4G11

Annotation: glutamine-synthetase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 986 PF03710: GlnE" amino acids 68 to 306 (239 residues), 211.4 bits, see alignment E=2.3e-66 amino acids 602 to 838 (237 residues), 216.3 bits, see alignment E=7e-68 PF08335: GlnD_UR_UTase" amino acids 364 to 486 (123 residues), 109.6 bits, see alignment E=1.9e-35 amino acids 876 to 949 (74 residues), 21.7 bits, see alignment 2.7e-08 PF03445: DUF294" amino acids 682 to 770 (89 residues), 21.1 bits, see alignment E=3.6e-08

Best Hits

Swiss-Prot: 68% identical to GLNE_RALSO: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 81% identity to reu:Reut_A1030)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y6U5 at UniProt or InterPro

Protein Sequence (986 amino acids)

>RR42_RS05890 glutamine-synthetase adenylyltransferase (Cupriavidus basilensis FW507-4G11)
MTSTDPTSSAAAPETAATDGAAGERAPGNTSGAARQAVADMATGPFAVVAAVELVPVDCA
GMALYSPAYSHYTRRMVQARPELAEIVAQARDQPLTRAWMAARLKVLHEPSADVDDAIKR
ALRRLRAEVMCVVIERDLRGQSTLGEVTGAMTDLAELGIQYGLAMLEADLAPTYGRPVGE
SSGEVQELVVVGMGKLGGRELNVSSDIDLIFLYDEDGDTRGGQRSLSNHEYFIRLGRRLI
NLLADVTADGYVFRVDMRLRPNGDAGPLACSFGMLEQYLMVQGREWERYAWIKGRVVSAL
DTPHAQRTAAQLASLTTPFVFRRYLDYGVIAAIRSLHAQIRAEAAKRSGGLAAPGGGQRV
NQRAFNIKLGRGGIREIEFMAQVFQLIRGGQDPALRIRPTLEVLAVAVEHGLLPAGQAEQ
LAHAYRFLRQLEHRLQYRDDAQTHHLPTSEEEQLAVAKMMGCADWPALLERLASLQDPVA
QQFEATFSDPDEAHAPCEALWPALIQEEQDEPPCERLASAGFSDCAGLLDRLRAIGGSLR
YRALSEPSRVRFDLLVNRALDLASRQEDADRTIARFVDFLDAISRRASYLSLLSEYPQAM
ARVADTLHASSWAAAYLTRHPQLLDELLDSDALAGAPDWAGFRSRLATRLDAADGQIEAQ
MDILRREHHAETFRILLQDLQGMLTVEQIADRLSDLADAMLEVTLQAVWRQLATRHAEQP
RFAVIAYGKLGGKELGYASDLDIIFLYEDEHERAPDVYAAYARRLITWLTSHTAAGTLFD
VDTRLRPNGEAGLLVTSFEGFRRYQQREGDNTAWVWEHQALTRARFCAGDPGIGAHFESL
RDEILRQPRDAATLRGEIVVMRHKVAEGHPNPSGLFDLKHDRGGMVDIEFTVQYLVLLHS
HDHPALTRNAGNIALLRTAGEIGLIDATLAREVGDAYRLFRARQHQLRLDGQVHARVAPA
VVAQQAEHVRTLWQAVFGEPVAAGGK