Protein Info for RR42_RS05485 in Cupriavidus basilensis FW507-4G11

Annotation: polynucleotide phosphorylase/polyadenylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 734 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 12 to 693 (682 residues), 1053.9 bits, see alignment E=0 PF01138: RNase_PH" amino acids 17 to 146 (130 residues), 102.6 bits, see alignment E=5.5e-33 amino acids 326 to 458 (133 residues), 104.7 bits, see alignment E=1.3e-33 PF03725: RNase_PH_C" amino acids 149 to 212 (64 residues), 47.3 bits, see alignment E=4.2e-16 amino acids 463 to 531 (69 residues), 23.9 bits, see alignment E=8.5e-09 PF03726: PNPase" amino acids 244 to 322 (79 residues), 62.4 bits, see alignment E=1.1e-20 PF00013: KH_1" amino acids 558 to 615 (58 residues), 45.5 bits, see alignment 1.3e-15 PF00575: S1" amino acids 621 to 692 (72 residues), 51.4 bits, see alignment E=2.8e-17

Best Hits

Swiss-Prot: 92% identical to PNP_CUPMC: Polyribonucleotide nucleotidyltransferase (pnp) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 93% identity to cti:RALTA_A1030)

MetaCyc: 61% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y691 at UniProt or InterPro

Protein Sequence (734 amino acids)

>RR42_RS05485 polynucleotide phosphorylase/polyadenylase (Cupriavidus basilensis FW507-4G11)
MTMFNKIVKEFQWGQHKVRLETGEVARQAGGAVIVDIEDTVVLATVVAAKSPKAGQDFFP
LTVDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
SLNPEVPADIPALIGASAALAVSGIPFSGPVGAARVGYKDGQYLLNPTRSQIAASELDLV
VAGTERAVLMVESEARQLSEEVMLGAVVYGHEQMQTAINAIHDLVRDGGKPEWDWTAAPK
NEALIAKVGEIALPLLQDVYQLRQKSARSQKLKEVYATVAAKLAEAGVEADKVEVGNVLF
DIEAKIVRSQVLSGEPRIDGRDTRTVRPIEIRSSVLPRAHGSAIFTRGETQALVVATLGT
KGDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKD
DEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPIKAHVAGVAMGLILEGNKFAVL
TDILGDEDHLGDMDFKVAGTDTGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQ
DAMGHARTELSEHAPRMITMKIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIA
STSTEGMAEAKRRIEGITAEAEVGKIYAGTVLKLLDFGAIVNILPGKDGLLHISEIVNER
VKDIKDWLKEGQHVRVKLIQADEKGRLRLSLKAALAEEGGSISPIANTSEAPAAPAAPAA
PAAPTTPSADQQQQ