Protein Info for RR42_RS04920 in Cupriavidus basilensis FW507-4G11

Annotation: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 TIGR00691: RelA/SpoT family protein" amino acids 109 to 798 (690 residues), 727.3 bits, see alignment E=9e-223 PF13328: HD_4" amino acids 109 to 258 (150 residues), 170.6 bits, see alignment E=6.8e-54 PF01966: HD" amino acids 128 to 227 (100 residues), 42.7 bits, see alignment E=1.9e-14 PF04607: RelA_SpoT" amino acids 318 to 428 (111 residues), 133.6 bits, see alignment E=1.1e-42 PF02824: TGS" amino acids 471 to 530 (60 residues), 70.6 bits, see alignment 2.8e-23 PF19296: RelA_AH_RIS" amino acids 543 to 700 (158 residues), 33.5 bits, see alignment E=1.4e-11 PF13291: ACT_4" amino acids 720 to 800 (81 residues), 58 bits, see alignment E=3.8e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 94% identity to cti:RALTA_A0952)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y0J1 at UniProt or InterPro

Protein Sequence (805 amino acids)

>RR42_RS04920 guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Cupriavidus basilensis FW507-4G11)
MTARTGAPNLPDPLGNDVVGERAVAPAMGAGKARAAQAAAESELSEAPQGAAPGDALFID
AVLAQSYRHFFGPTSQPAVPPRQQVISITRLMEKLAYLKPADQAHVREAFQFSDEAHLGQ
YRQSGEPYITHPVAVAELCADWKLDVQSIMAALLHDVMEDQGITKSELAEKFGPKVSELV
DGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDFVPPEKRRR
IALETMEIYAPIAHRLGLNTTYRELQELSFKVGSPFRYATLEKAVKAARGNRREVVKRIL
EAAQKALGDAGIVAELSGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMHTYMAM
GALHGLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTRDMHQIAEAGVAA
HWMYKHQADQANDIQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPKGHI
RALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKSGDIVEVVTAPYSKPNPAW
LAFVRTGKARAAIRHYLKTTRLDEAIQLGERLLEQSARQLGIELKALPQSVWDRMVQWTG
NKHKEDIFADLALGRRVPAVVAKRMEILLQELSGDVDSALLAAVQTFAGEEAPAVPITGD
EGMSMIFSSCCRPIPGDSIVGYLGKGEGLQIHVQDCKIAKRLHSKDPEHWIDVMWAKKTT
RSFDVSIKVMVRNVKGIVARVAADLTSADANVAHVAMEQQEGGHQEATYMQFIIQVQNRL
HLANVMRALRRNPDVIRIFRDRNDG