Protein Info for RR42_RS04445 in Cupriavidus basilensis FW507-4G11

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 904 TIGR01063: DNA gyrase, A subunit" amino acids 10 to 855 (846 residues), 1201 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 513 (482 residues), 555 bits, see alignment E=1.3e-170 PF03989: DNA_gyraseA_C" amino acids 545 to 592 (48 residues), 40.1 bits, see alignment 1.9e-14 amino acids 595 to 644 (50 residues), 38.7 bits, see alignment 5.4e-14 amino acids 652 to 696 (45 residues), 32.6 bits, see alignment 4.3e-12 amino acids 700 to 745 (46 residues), 48 bits, see alignment 6.7e-17 amino acids 759 to 805 (47 residues), 44.7 bits, see alignment 7e-16 amino acids 808 to 851 (44 residues), 44.1 bits, see alignment 1.1e-15

Best Hits

Swiss-Prot: 62% identical to GYRA_SALTY: DNA gyrase subunit A (gyrA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 85% identity to bmv:BMASAVP1_A2578)

MetaCyc: 61% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y5Q3 at UniProt or InterPro

Protein Sequence (904 amino acids)

>RR42_RS04445 DNA gyrase subunit A (Cupriavidus basilensis FW507-4G11)
MDQFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMHELNNDWN
RPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYMLVDGQGNFGSVDGDNAAAM
RYTEIRLSKIAHEMLLDIDKETVDFGPNYDGSEHEPLILPARIPNLLINGSSGIAVGMAT
NIPPHNLNEVVDGCLHLLRNPQATIDELIELIPAPDFPTAGIIYGIQGVREGYRTGRGRV
VMRAKTHFEDIDRGQRQAIIVDELPYQVNKRTLLERIAELVTDKKVEGISDIRDESDKSG
MRVVIELKRNEVPEVVLNNLYKHTQLQDTFGMNMVALVDGQPRLLNLRQMLDCFLSHRRE
VVTRRTVFELRKARERGHVLEGLAVALANIDEFIAIIKAAPTPPIAKQGLMAKAWDSSLV
REMLARAEGDTAGGRASYRPDGLPAIFGMQADGLYRLSDSQAQEILQMRLQRLTGLEQDK
IVQEYRDVMAVISDLLDILSRPERISTIITDELTAIRAEFGDVRRSHIELNATELDTEDL
ITPQDMVVTLSHSGYMKSQPISEYRAQKRGGRGKLATTTKEDDWIDTLFVANTHDYILCF
SNRGRLYWLKVYEVPQGSRNSRGRPIVNMFPLSDGEKINVILPVRQFDAEHFIFMSTARG
TVKKTALTEFSNPRKAGIIAVDLDEGDYLIGAAVTDGQHDVMLFSDSGKAVRFDENDVRP
MGRQARGVRGMNLEESQTVIAMLVAPAETSEQAVAVGSVLTATENGYGKRTPIAEYTRHG
RGTKGMIAIQTSERNGRVVAAALVSPEDEIMLITTGGVLIRTRVSEIREMGRATQGVTLI
NVDEGTKLSGLQRIVESDADNGVAGDELEEGADAVDAAAPAADGEQAGAQDGTDAGTPDT
GSNS