Protein Info for RR42_RS03760 in Cupriavidus basilensis FW507-4G11

Annotation: formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 951 PF13510: Fer2_4" amino acids 18 to 94 (77 residues), 84.7 bits, see alignment E=1.7e-27 PF10588: NADH-G_4Fe-4S_3" amino acids 101 to 140 (40 residues), 60.3 bits, see alignment (E = 4.7e-20) PF13237: Fer4_10" amino acids 162 to 221 (60 residues), 25.1 bits, see alignment 6.3e-09 PF12838: Fer4_7" amino acids 166 to 223 (58 residues), 33.9 bits, see alignment 1.6e-11 PF00037: Fer4" amino acids 208 to 223 (16 residues), 23.5 bits, see alignment (E = 1.7e-08) PF04879: Molybdop_Fe4S4" amino acids 239 to 288 (50 residues), 54.9 bits, see alignment 3.1e-18 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 242 to 917 (676 residues), 930.6 bits, see alignment E=2.2e-284 PF00384: Molybdopterin" amino acids 294 to 712 (419 residues), 233 bits, see alignment E=2.4e-72 PF01568: Molydop_binding" amino acids 809 to 914 (106 residues), 107 bits, see alignment E=2.4e-34

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 82% identity to bph:Bphy_0727)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YC49 at UniProt or InterPro

Protein Sequence (951 amino acids)

>RR42_RS03760 formate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MNTRNEIDFGTPASDSQQQVTLEIDGVDVTVPAGTSLMRAAVQAGVNVPKLCATDSLKSF
GSCRLCLVEIEGRRGFPASCTTPAEAGMKVKTQTDKLADLRRGVMELYISDHPLDCLTCP
TNGNCELQDMAGVVGLREVRYGYDGANHRAEAKDESNPYFTYDPSKCIVCNRCVRACEET
QGTFALTISGRGFDARVTAGQGDSFMDSDCVSCGACVQACPTATLAETSIIRMGQPEHSK
VTTCAYCGVGCAFKAEMKGNEVVRMVPYKNGQANEGHSCVKGRFAFGYATHKDRILNPMI
RARITDPWREVSWEEAISHAASEFKRIQAKHGREAIGGIVSSRCTNEEGYLVQKLVRAAF
GNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFRSVEKADVIMVMGANPTDGHPVFASRM
KKRLRAGAKLIVIDPRRIDLVDSPHVRADFHLQLRPGTNVAVVTSMAHVIVSEGLMAESF
IAERCEDRAFQQWRDFVMLPENSPEAMAEITGIPAGQLRGAARLYATGGNAAIYYGLGVT
EHAQGSTTVMGIANLAMCTGNIGREGVGVNPLRGQNNVQGSCDIGSFPHELPGYRHVSDS
TTRGLFESAWNVEISPEPGLRIPNMFEAALSGSFMGLYCQGEDIVQSDPNTQHVSAALSA
MECIVVQDIFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPPKARYADWEAT
VLLSNALGYPMDYKHPSEIMDEIARLTPTFSGVSYQRLEELGSIQWPCNDAAPEGTPTMH
IDAFLRGKGKFIITRYVPTNERVNRKFPLILTTGRILSQYNVGAQTRRTENVMWHDEDRL
EVHPHDAEERGIKDGDWVGVQSRAGETVLRAIVSERMQPGVVYTTFHFPESGANVITTDN
SDWATNCPEYKVTAVQVMPVAQPSEWQRQYRDFHDQQMQLLQAASAEAGPA