Protein Info for RR42_RS01780 in Cupriavidus basilensis FW507-4G11

Annotation: glutathione synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 PF02951: GSH-S_N" amino acids 1 to 119 (119 residues), 148 bits, see alignment E=1.8e-47 TIGR01380: glutathione synthase" amino acids 1 to 311 (311 residues), 416.2 bits, see alignment E=3.5e-129 PF02955: GSH-S_ATP" amino acids 123 to 296 (174 residues), 250.6 bits, see alignment E=9.4e-79 PF08443: RimK" amino acids 155 to 307 (153 residues), 39.4 bits, see alignment E=7.4e-14

Best Hits

Swiss-Prot: 76% identical to GSHB_RALSO: Glutathione synthetase (gshB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 84% identity to reu:Reut_A0294)

MetaCyc: 48% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YB37 at UniProt or InterPro

Protein Sequence (317 amino acids)

>RR42_RS01780 glutathione synthetase (Cupriavidus basilensis FW507-4G11)
MRILFITDPLDTFKTYKDSTYAMMVEAVERGHELFACLQSQLSLSAHQVEAVVRPLQLTG
DAHDWYRAGEPGLQPLTGFDAVVMRKDPPFDMEYVTSTWLLELAESQGANVFNKPQAIRD
HSEKLAIARYPEYITPTLVTRDADRIRAFHAEHRDVILKPLDGMGGMGIFRVGAEGLNLG
AIIETLGENGARTLMVQRYIPEIKDGDKRILLIGGVPVPYSLARIPKDGEVRGNLAAGGT
GRAQELSARDREIAEALAPGLWEQGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFQV
AAMFIDALEHAVQARSR