Protein Info for RR42_RS01600 in Cupriavidus basilensis FW507-4G11

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 32 to 145 (114 residues), 57.6 bits, E=1.3e-19
• PF02310: B12-binding
  • amino acids 34 to 138 (105 residues), 53.1 bits, E=5.6e-18
• PF03308: MeaB
  • amino acids 186 to 390 (205 residues), 141.3 bits, E=6.5e-45
• PF02492: cobW
  • amino acids 216 to 386 (171 residues), 31.9 bits, E=2e-11
• PF01642: MM_CoA_mutase
  • amino acids 578 to 1095 (518 residues), 418.4 bits, E=8.2e-129

Best Hits

Swiss-Prot: 87% identical to ICMF_CUPMC: Fused isobutyryl-CoA mutase (icmF) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K11942, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 74% identity to afw:Anae109_3169)

Predicted SEED Role

"B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• methylaspartate cycle (14/19 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (10/18 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See A0A0C4Y4J6 at UniProt or InterPro

Protein Sequence (1102 amino acids)

>RR42_RS01600 methylmalonyl-CoA mutase (Cupriavidus basilensis FW507-4G11)
MTDLSDVQKGAQPGAQPGAPHQAQGPKGPVNKVRFVTAASLFDGHDASINIMRRILQSSG
CEVIHLGHNRSVEEVVSAALQEDVQGIAISSYQGGHVEYFKYMVDLLRRRGGEHIQLFGG
GGGVIVADEIRELQAYGVARIFSPEDGQRLGLAGMIADMVQRCDIDLARYAPATLDAMKG
GDRRALAQLITALENGKADPALADTLRAEAATVKTPVLGITGTGGAGKSSLTDELIRRIR
LDQRDALSIAVISIDPSRRKSGGALLGDRIRMNAINHPHIFMRSMATREAGSEISRALPD
VLAACKVAGFDLIIVETSGIGQGDAAIVAHVDLSLYVMTPEFGAASQLEKIDMLDFADFV
AINKFDRKGAQDAWRDVAKQVQRNREQWHAKPEEMPVYGTQASRFNDDGVTMLYQGLREA
LCARGMRAPAGVLPQLSGRVSTGQNVIVPPARNRYLAEIADTVRAYHRRVDAQSRLARER
QQLREARRMLLAASGQEDQGEALQALATERDAALGAQERKLLAMWPQMRKAYSGDEYVVK
IRDKEIRTTLTTTTLSGTTVRKVVLPRFEDDGEVLKWLLRENVPGSFPYTAGVFAFKREN
EDPTRMFAGEGDAFRTNRRFKLVSEGMDAKRLSTAFDSVTLYGEDPHTRPDIYGKVGNSG
VSIATLDDMKVLYDGFDLTSPSTSVSMTINGPAPTILAMFMNTAIDQQFDKFRADNDREP
TVDEQGKIRAWVLQNVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIQAYFVHHQVRNF
YSVSISGYHIAEAGANPISQLAFTLSNGFTYVEAYLARGMHIDDFAPNLSFFFSNGMDPE
YSVLGRVARRIWAVTLRDKYGANERSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALIAI
YDNCNSLHTNAYDEAITTPTGESVRRALAIQLIINREWGVARCENPNQGSFLIDELTDLV
EDAVMQEFERIAERGGVLGAMETGYQRGKIQDESLYYEQRKHDGTLPIIGVNTFRNPDGD
AAPPSLELARSSEEEKQGQLARLADFHARHADAAPAMLQRLRQAVIDNGNVFAVLMDAVR
VCSLGQVTHALFEVGGQYRRNM