Protein Info for RR42_RS00955 in Cupriavidus basilensis FW507-4G11
Annotation: hydroxymethylglutaryl-CoA lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to HMGCL_MOUSE: Hydroxymethylglutaryl-CoA lyase, mitochondrial (Hmgcl) from Mus musculus
KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 91% identity to reh:H16_A0186)MetaCyc: 58% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]
Predicted SEED Role
"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)
MetaCyc Pathways
- L-leucine degradation I (5/6 steps found)
- ketogenesis (4/5 steps found)
- mevalonate degradation (1/2 steps found)
- cis-geranyl-CoA degradation (4/9 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Geraniol degradation
- Synthesis and degradation of ketone bodies
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.4
Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4XYP7 at UniProt or InterPro
Protein Sequence (311 amino acids)
>RR42_RS00955 hydroxymethylglutaryl-CoA lyase (Cupriavidus basilensis FW507-4G11) MTLPHYVKVVEVGPRDGLQNEKAMVPTEVKVDLINRLSDAGFVNIEAASFVSPKWVPQMA DGADVMARIQRRPGTLYSVLTPNMKGFEGAVAASADEVVIFGAASEAFSQKNINCSIAES IERFAPVAAAAKEKGLRLRASISCALGCPYQGEVPVHAVVDVVRRMRDLGCDEIDIADTI GVGTPGRVQEVMHAVAAEFPIDRLSGHFHDTYGQALSNILASLQVGISIFHASVAGLGGC PYAKGATGNVATEDVLYMLHGMGIHTGVDLEQVVRAGDFISQSIGRANSSRVGRALLTKW AAAADAAPACV