Protein Info for RR42_RS00875 in Cupriavidus basilensis FW507-4G11

Annotation: isocitrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 614 PF20423: AceK_regulatory" amino acids 17 to 322 (306 residues), 393.1 bits, see alignment E=8.5e-122 PF06315: AceK_kinase" amino acids 323 to 582 (260 residues), 381.4 bits, see alignment E=2.2e-118

Best Hits

Swiss-Prot: 89% identical to ACEK_CUPNH: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 89% identity to reh:H16_A0168)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y667 at UniProt or InterPro

Protein Sequence (614 amino acids)

>RR42_RS00875 isocitrate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MSHFPKLLSSQIAYDVARTMLDGFDKHYRLFREAGHLAKLKFEAADWPGLQQLQRDRIDF
YNERVREASVILEDEYDAENIEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVCTRILHR
SYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSRKGMAACFERIVHNFQLERPFDD
LTRDIGYVVRAVTEYFGDFRIAPNFQIHVLSSLFFRNKSAFIIGRILNADRTFPLAIPIV
HTPDGKLSLDTVLLKKVQLLILFSFTHSYFMVDMEIPSAYVTFLRDIMPRKPRAEIYTSL
GLQKQGKNLFYRDFLHHLQHSSDNFISAPGIKGLVMLVFTLPSYPYVFKVIKDYYPAPKE
TTRELIKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDDALVRELELHAPSMIEYQRGK
DGGEEIVVRHVYIERRMTPLNIWLQEGSDAQVEHGIVEYGNAIKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYMTDTNVRHVPQPRNEEEELSGEVWYSVGRHDIFPETYRTFL
LGDARVRAAFLRHHADFFDPAMWQAHKDRLLAGQIHDFYAYDVSERFIHRYAAGSGDAEA
RAHGNDPVPARRVA