Protein Info for RR42_RS00365 in Cupriavidus basilensis FW507-4G11

Annotation: dihydropyrimidinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 TIGR02033: dihydropyrimidinase" amino acids 8 to 464 (457 residues), 523.5 bits, see alignment E=2.6e-161 PF01979: Amidohydro_1" amino acids 53 to 445 (393 residues), 100.2 bits, see alignment E=1.4e-32 PF07969: Amidohydro_3" amino acids 366 to 425 (60 residues), 30.5 bits, see alignment E=2.9e-11

Best Hits

Swiss-Prot: 43% identical to HYDA_STRCO: D-hydantoinase (hyuA) from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 91% identity to rpf:Rpic12D_4135)

MetaCyc: 43% identical to dihydropyrimidinase (Brevibacillus agri)
Dihydropyrimidinase. [EC: 3.5.2.2]; 3.5.2.2 [EC: 3.5.2.2]

Predicted SEED Role

"D-hydantoinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism (EC 3.5.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.2

Use Curated BLAST to search for 3.5.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4XYG5 at UniProt or InterPro

Protein Sequence (471 amino acids)

>RR42_RS00365 dihydropyrimidinase (Cupriavidus basilensis FW507-4G11)
MNTELDLVIRNAEVVTASDRFTCDIGVRDGRIAMLGHDLPRAAREMDATGLLALPGGVDG
HVHLDQPMPDGMRMADDFFTGTRAAVCGGTTTVIPFAAQEKGGSLKAAVADYHRRAEGRA
VADYGFHLIVADPTPDVLQHELPELIRKGYTSFKVYMTYDDLKLSDREMLDVLDVAKRND
ALVMVHAENADCISWLTEKLVGQGRISPRFHGMARPALVEREATHRAITFAELVDVPILI
VHVSGKEAIEQIRWAQGRGLPILAETCPQYLYLTAEDMDLPGDDGYEGAKCVCSPPPRDA
ENQEAVWRALANGVFSVFSSDHAPFNYDDPQGKKLGGSAQPFDHIPNGVPGIETRLPLLF
SGIAQGRLSLHQFVELTSYRPARLYGLYPRKGTIAVGADADIALWDPSRKVRIANSALHH
AVDYTPYEGIEVTGWPVHCFSRGELLVEDGRYLEPVPGRGKFLPAGAPSLV