Protein Info for RR42_RS00015 in Cupriavidus basilensis FW507-4G11

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 845 TIGR01059: DNA gyrase, B subunit" amino acids 17 to 845 (829 residues), 946.3 bits, see alignment E=4.2e-289 PF02518: HATPase_c" amino acids 44 to 152 (109 residues), 55.6 bits, see alignment E=2e-18 PF00204: DNA_gyraseB" amino acids 253 to 422 (170 residues), 191.3 bits, see alignment E=2.9e-60 PF01751: Toprim" amino acids 451 to 563 (113 residues), 66.9 bits, see alignment E=4.5e-22 PF21249: GyrB_hook" amino acids 565 to 593 (29 residues), 35.4 bits, see alignment (E = 1.9e-12) PF18053: GyrB_insert" amino acids 599 to 771 (173 residues), 142.6 bits, see alignment E=3.4e-45 PF00986: DNA_gyraseB_C" amino acids 774 to 834 (61 residues), 95.4 bits, see alignment 5.5e-31

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 73% identity to bac:BamMC406_0003)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y3N6 at UniProt or InterPro

Protein Sequence (845 amino acids)

>RR42_RS00015 DNA gyrase subunit B (Cupriavidus basilensis FW507-4G11)
MTEEQQPQSQPQQQDSYGAGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSI
DEALAGWCTEITVTIHSDNSISITDNGRGIPPLVKFDDKHEPKRSAAEIAMTELHAGGKF
NQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGQAHLLEFAQGAVQNRLVETVTGPDGQ
PVEVSPMKILGPTDKRGTEVHFLADEEIFTNVEFHYEILAKRIRELSFLNNGVHIRLADQ
RTGKEEDFAFSGGVKGFVEYINKGKSTLHPNIFYANTEKDGIAVEVSMQWNDGYNEQVLC
FTNNIPQRDGGTHLTGLRAAMTRVMNKYIEENEIAKKAKVETTGDDMREGLACVLSVKVP
EPKFSSQTKDKLVSSEVRLPVEELVAKALGDFLLETPVDAKIICGKIVDAARAREAARKA
REMTRRKGVLDGMGLPGKLADCQEKDPAQSELFLVEGDSAGGSAKQGRDRKFQAILPLKG
KILNVERARFDKMLSSQEVLTLITALGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRT
LLLTFFYRQMPDIIERGHVYIAQPPLYKIKHGKEERYIKDDNELNAYLLRLAMEKAALVN
PDGSEVAGDALTELARQYQLAEAVIGRLARIVDSDALRAIADGIELDLDSAAAAEASATA
LKAKLSEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHLDADFVH
GADYAALSLAAKTFQGLIAEGTKVRRGEGEKMRDQTVNDFHAAMQWLLSEAERGVSRQRY
KGLGEMNPEQLFETTLDVTQRRLLKVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVA
RNIDV