Protein Info for RALBFv3_RS24300 in Ralstonia solanacearum IBSBF1503

Annotation: 4-hydroxythreonine-4-phosphate dehydrogenase PdxA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 769 transmembrane" amino acids 97 to 114 (18 residues), see Phobius details PF07005: SBD_N" amino acids 11 to 226 (216 residues), 165.7 bits, see alignment E=2.2e-52 PF17042: NBD_C" amino acids 253 to 402 (150 residues), 67 bits, see alignment E=4.7e-22 TIGR00557: 4-hydroxythreonine-4-phosphate dehydrogenase PdxA" amino acids 436 to 757 (322 residues), 360.3 bits, see alignment E=5.5e-112 PF04166: PdxA" amino acids 462 to 753 (292 residues), 348.5 bits, see alignment E=4.8e-108

Best Hits

KEGG orthology group: K00097, 4-hydroxythreonine-4-phosphate dehydrogenase [EC: 1.1.1.262] (inferred from 84% identity to rpi:Rpic_0564)

Predicted SEED Role

"4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.262)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.262

Use Curated BLAST to search for 1.1.1.262

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (769 amino acids)

>RALBFv3_RS24300 4-hydroxythreonine-4-phosphate dehydrogenase PdxA (Ralstonia solanacearum IBSBF1503)
MNAAAQAGPKLLILADDLSGAADCAVAGVRAGLRSAVMLRADGAPPAADVLALDTDTRRA
GAPDAAARQLAAWQALARPATRLYKKIDSTLRGNVAAEVAALVPVAGLAVVAPAFPAAGR
TTRNARQWLHGVAVEETEVWRNEGIAGRADLLDMLVRQGLRAAALSLADIRAGAAVLAGR
LASLQADGVRAVVCDCETDDDLAHIADASVRLAHAFWVGSAGLAPTLIRALGLGSANAAA
GADTAAPATGPILTVVGSMSSVSHAQANDLTATAGGALLALELPIDALDTPQAGLTQRVI
DALCEGRDVLVTLSQATRGSSADGLRFCRRLAALLAPALPHAGGLVATGGETARALLAAA
GIDALQLADEVEPGMPLLHARLAGRALPVVTKAGGFGGPAALSCAWRRLAGAERADPASL
TTLSKGNPAMTYRPVIGITMGDAAGVGPEIIMKALTHRSVYEQCRPLVIGDTARLRDAGR
RAGVSLEVRSIERPADAAFRYGVVDCIDLGLIPADLPYGQLSPIAGDAAYQYIARTVELT
SAGELDAICTAPLNKEALHAGGHLFPGHTEMLAHLTGIDEVSMMLVAPNLRVIHVTTHIG
LLDAIRRIEPGLVQRTIERAHATLVRAGIGNPRIGVCGINPHAGENGLFGYGEEEEKIVP
AVQVLQARGWRVEGPLPADTLFFRAGRGDFDVVVAMYHDQGHGPVKVMGLEAGVNVTVGL
PVIRTSVDHGTAFDIAGKGIADERSLLEAFRQACELATRHDEPKTARAA