Protein Info for RALBFv3_RS23085 in Ralstonia solanacearum IBSBF1503

Annotation: nitric-oxide reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 229 to 250 (22 residues), see Phobius details amino acids 283 to 306 (24 residues), see Phobius details amino acids 333 to 354 (22 residues), see Phobius details amino acids 366 to 385 (20 residues), see Phobius details amino acids 411 to 432 (22 residues), see Phobius details amino acids 444 to 465 (22 residues), see Phobius details amino acids 480 to 505 (26 residues), see Phobius details amino acids 517 to 538 (22 residues), see Phobius details amino acids 544 to 565 (22 residues), see Phobius details amino acids 585 to 608 (24 residues), see Phobius details amino acids 628 to 651 (24 residues), see Phobius details amino acids 664 to 689 (26 residues), see Phobius details amino acids 715 to 736 (22 residues), see Phobius details PF00115: COX1" amino acids 279 to 722 (444 residues), 194.6 bits, see alignment E=1.5e-61

Best Hits

KEGG orthology group: K04561, nitric oxide reductase, cytochrome b-containing subunit I [EC: 1.7.2.5] (inferred from 90% identity to rpi:Rpic_4013)

MetaCyc: 49% identical to nitric oxide reductase (menaquinol) (Cupriavidus necator H16)
RXN-12129 [EC: 1.7.5.2]

Predicted SEED Role

"Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent" in subsystem Denitrification or Flavohaemoglobin or Nitrosative stress (EC 1.7.99.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.5 or 1.7.5.2 or 1.7.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (758 amino acids)

>RALBFv3_RS23085 nitric-oxide reductase large subunit (Ralstonia solanacearum IBSBF1503)
MKTNHGLWRWLGLIFVLSFGALGYLGRQIYLAAPPIPQAVVTTAGDVLFTGEQVERGQQA
WLSAGGQQLGTVWGHGSYVAPDWSADWLHREATALQGIRAHQQYGAAGPLTAPEQAAVDA
AVKEEMRRNTYDTSHGSVTVSKERALAIREVAQHFEALFGTDPSLAELRSQYAMATGALP
AQADREALAAFFFWTSWAAATDRPGETDLSYTSNWPHEPLVGNTMTGGAAMWSMVSIILL
LAGIAAMLWMHGAGKHEEEAQPLASDPFLNVVATPSMKATRKYFFAVVGLMLLQIGMGAI
TAHYAVEGEAFFGIPLAQVLPYVISRTVHTQLGVLWIATAWLATGLYIAPLLSGREPKFQ
KLGVDLLFWALIFIVVGSIVTGWLGTLQHRGVDFSFWLGNQGLEYTSMGRVWQLLLFVGL
LFWVVLLGRALWPALKTPSESRGLIAMVFLSATCIGGFYSTSLVWDQHTHYSMIEYWRWW
LVHLWVEGFFEVFATAVVALIFTRLGLIRAASANRAIVAETIVFLFGGILGTLHHLYFTG
TPTSIIAVGAVFSALEVVPLTLIGLEALQNYRRTQGAAWLGAYKWPVLCFVAVGFWNTVG
AGLLGFAINPPASLYYVQGLNMTAAHGHAALFGVYGMLGIGLMLFCLRGLYARSLHADRL
LKPAFWGLNIGLAMMVFMSLLPAGIYQAWASVTKGMWYARSPEIVHSKVMETLVWLRVPG
DIVFAAGAVLLAWYALRLLRRPNARQPEPVAVSANATR