Protein Info for RALBFv3_RS19915 in Ralstonia solanacearum IBSBF1503

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1081 TIGR00594: DNA polymerase III, alpha subunit" amino acids 21 to 1026 (1006 residues), 501.6 bits, see alignment E=2.5e-154 PF02811: PHP" amino acids 22 to 177 (156 residues), 60.4 bits, see alignment E=7.4e-20 PF07733: DNA_pol3_alpha" amino acids 307 to 561 (255 residues), 290.8 bits, see alignment E=2.7e-90 PF17657: DNA_pol3_finger" amino acids 564 to 741 (178 residues), 172.5 bits, see alignment E=1.2e-54 PF14579: HHH_6" amino acids 818 to 910 (93 residues), 70.8 bits, see alignment E=2.2e-23 PF01336: tRNA_anti-codon" amino acids 994 to 1064 (71 residues), 27.3 bits, see alignment 7.3e-10

Best Hits

Swiss-Prot: 93% identical to DNAE2_RALSO: Error-prone DNA polymerase (dnaE2) from Ralstonia solanacearum (strain GMI1000)

Predicted SEED Role

"macromolecule metabolism; macromolecule synthesis, modification; dna - replication, repair, restr./modif."

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1081 amino acids)

>RALBFv3_RS19915 error-prone DNA polymerase (Ralstonia solanacearum IBSBF1503)
MNASTPPPIGTIGPIGLPDYAELHCISNFTFLTGASHPHELVARAKVLGYWALALTDECS
VAGTVRAHVAAKEAGLKLIVGSRFTVRDAQGEPWLRLVLLAQDIDGYGNLCECITQARLA
APKGDYRLLADDLAAPAAGLAHLRGMPHCLAILLPDYDPDPQRLRAQALWCRQVFGDRLS
MALELPLRHADDRHRGTVAAVSEQADVPLVATGDVAMHSRQRKPLHDVLTAIRLSRPIAE
CGLELAPSAEQAMRTRMQLAFFYQGERGAPLLRRSVELASLCDFSLDEIEYEYPHEVVPE
GLTPAAYLHAEVMAGAARRYGKAIPAKVQAQIAEELALIAELRYEPFFLTVYDVVRFARS
RDILCQGRGSAANSAVCYCLGITEVDPESGNTLFARFLSRARNEPPDIDVDFEHQRREEV
IQYIYGKYGLTRTALAAALITYRTRSALRDVGRALGIDLGVIEQVAKAQAWWDGQHAFAQ
HAGQQGLDPESPLVQRWAGLVGQLRGFPRHLSQHVGGFVIAQGKLSRLVPIENAAMRDRR
VIQWDKDDLESLRLLKVDVLALGMLTAIRRTLDMLDALPGRRARYGASDKLAMQHLPGED
RDTYDMISRAETIGVFQIESRAQQSMLPRLRPRTYYDLVIQVAIVRPGPIQGGMVHPYLQ
RREAMRNGNPDCVTYPGPEVRAVLERTLGVPIFQEQVMEIAMKAGGFTADEADRLRRDMA
AWKRKGNLTEHQERLMKEMVEVRHYDPAFVEALCRQMEGFAEYGFPESHAAGFARLAYVS
SYLKCHEPAAFFAALLNSQPMGFYSPSQLVQEARRSRVRVLPADVTVSAWDSTLHERPPG
AGGQPDIRLGLSRIRGMRPSAAERIVAARAQAPFASVEDLAHRAVLDRHDLSVLAAANAL
LPLAGHRRQALWQTLALQEPGQDHALLRQARPVEAPLALPAPSLGEEVVADYGSLGLSLQ
SHPLSLLRPRLERMRFVTAATLAGYRNGQLARACGIVTVRQRPGTAKGTIFVSIEDETGV
VNVVLWPRLIERQRREVLHARLLGVYGAWQCERETRHLVAQHLVDLTPLLGRLASSSRDF
H