Protein Info for RALBFv3_RS18945 in Ralstonia solanacearum IBSBF1503

Annotation: NAD-dependent succinate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 21 to 479 (459 residues), 553.9 bits, E=1.2e-170
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 31 to 477 (447 residues), 663 bits, E=1.1e-203

Best Hits

Swiss-Prot: 54% identical to SLAD_PSEPU: 3-sulfolactaldehyde dehydrogenase (PpSQ1_00395) from Pseudomonas putida

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 94% identity to rsl:RPSI07_mp1031)

MetaCyc: 52% identical to Succinate-semialdehyde dehydrogenase, mitochondrial (Homo sapiens)
Succinate-semialdehyde dehydrogenase. [EC: 1.2.1.24]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt I (3/4 steps found)
• GABA shunt II (3/4 steps found)
• methylglyoxal degradation IV (2/3 steps found)
• methylglyoxal degradation V (2/3 steps found)
• superpathway of 4-aminobutanoate degradation (2/3 steps found)
• L-lactaldehyde degradation (aerobic) (1/2 steps found)
• superpathway of methylglyoxal degradation (5/8 steps found)
• 4-aminobutanoate degradation IV (1/3 steps found)
• N-methylpyrrolidone degradation (1/3 steps found)
• superpathway of L-arginine and L-ornithine degradation (8/13 steps found)
• lactate biosynthesis (archaea) (2/5 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (6/11 steps found)
• L-rhamnose degradation II (2/8 steps found)
• superpathway of fucose and rhamnose degradation (3/12 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Pyruvate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.22

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.22 or 1.2.1.24

Protein Sequence (485 amino acids)

>RALBFv3_RS18945 NAD-dependent succinate-semialdehyde dehydrogenase (Ralstonia solanacearum IBSBF1503)
MPLTLDRTDLVRSANYIAGTWTAAAGPLLDVTDPATGELITRVPDSGAAEARAALDAAQA
AFAAWRKVPAKLRAQIIKRWNDLVLAHQDDLGKLISREQGKPLAEGKGEVAYAASYIEWF
GEEATRMNGETIPAPVPGRRMFALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVC
KPAEDTPLTSLALVRLAEEAGVPAGVLNIVTASRERTPEVVDVWLDDPRVRKITFTGSTP
VGKHLARRSAETLKKLSLELGGNAPFIVFEDADLDAAVDGFMAAKFRNGGQTCVCPNRVF
VHDAFAARLAARVEALKVGPASDPASQIGPMINARAVEKIERHVQDAVARGARVLTGGRR
LTELGPNYYAPTVLTHADATMACACEETFGPVAPLTRFTTEAEVIAAANDTPFGLAAYFY
SQDVRRIWRVADQLETGIVGVNEGAVASEAAPFGGVKESGYGREGSVHGLDDYLHTKYVC
QGQLD