Protein Info for RALBFv3_RS16735 in Ralstonia solanacearum IBSBF1503

Annotation: NarK family nitrate/nitrite MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 29 to 53 (25 residues), see Phobius details amino acids 65 to 83 (19 residues), see Phobius details amino acids 95 to 114 (20 residues), see Phobius details amino acids 121 to 147 (27 residues), see Phobius details amino acids 162 to 188 (27 residues), see Phobius details amino acids 209 to 228 (20 residues), see Phobius details amino acids 251 to 274 (24 residues), see Phobius details amino acids 286 to 304 (19 residues), see Phobius details amino acids 314 to 335 (22 residues), see Phobius details amino acids 342 to 362 (21 residues), see Phobius details amino acids 398 to 417 (20 residues), see Phobius details amino acids 432 to 452 (21 residues), see Phobius details TIGR00886: nitrite transporter" amino acids 27 to 413 (387 residues), 347.1 bits, see alignment E=6.3e-108 PF07690: MFS_1" amino acids 36 to 373 (338 residues), 56.6 bits, see alignment E=1.1e-19

Best Hits

Swiss-Prot: 52% identical to NARK_ECOLI: Nitrate/nitrite transporter NarK (narK) from Escherichia coli (strain K12)

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 95% identity to rsl:RPSI07_mp1510)

MetaCyc: 52% identical to nitrate:nitrite antiporter NarK (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-239

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>RALBFv3_RS16735 NarK family nitrate/nitrite MFS transporter (Ralstonia solanacearum IBSBF1503)
MASNVLTHWEPENPVFWHATGQPVAYRNLWISIPALMIAFAIWSLWSVVVVNLDKAGFAL
TKNQMFWLTALPALSGATLRIFYSFLVPIFGGRRWTALSTASLLIPALGMGFALRDPQTS
YPTLLVLALLCGLGGGNFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSAVQFITPLVI
SVGVFGTLAGDPQVFTAGGVQRSLWLQNAGFLWVPFICAATLAAWFGMHDIADAKASFSE
QAVIFKRTHNWLMCLLYVGTFGSFIGFSAGLALLTKSQFPDIDPTAYVFLGPLAGAITRP
VGGWISDKMGGARVTLWTFAGMIGGVAGVLMFLPSAGNPGNFAGFLAMFIVLFALTGIGN
GSTFRMIPIIFLTQHQRAAQGKGDAALRQATQDATRESAAVLGFSGAIGAYGGFFIPKSF
GTSLDLTGTPSAALYCFIGFYVVCMLVTWLVYARRNAPMPC