Protein Info for RALBFv3_RS15065 in Ralstonia solanacearum IBSBF1503

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00464: glutamate--tRNA ligase
  • amino acids 5 to 464 (460 residues), 550.2 bits, E=2.3e-169
• PF00749: tRNA-synt_1c
  • amino acids 5 to 311 (307 residues), 329.4 bits, E=1.8e-102
• PF19269: Anticodon_2
  • amino acids 325 to 464 (140 residues), 97.6 bits, E=9e-32

Best Hits

Swiss-Prot: 95% identical to SYE_RALSO: Glutamate--tRNA ligase (gltX) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 98% identity to rsc:RCFBP_20254)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
• tRNA charging (20/21 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• superpathway of heme b biosynthesis from glutamate (9/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (465 amino acids)

>RALBFv3_RS15065 glutamate--tRNA ligase (Ralstonia solanacearum IBSBF1503)
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGDFILRIEDTDVERSTQEAVDVIL
EAMEWLGLDVDEGPFYQMQRMDRYREVIAQMLEQGLAYPCYMSTEELDALREAQRVRGEK
PRYDGTWRPEPGKILPAPPAGVQPVIRFKNPIGGSVVWDDAVKGGIEISNDELDDLVIAR
PDGTPTYNFCVVVDDMDMRITHVIRGDDHVNNTPRQINILRALGGTTPVYAHLPTVLNEQ
GEKMSKRHGAMSVTGYRDAGYLPEAVVNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKS
PAQYNPEKLAWLNNHYIKQADNERLAGLTKPFIEALGGKVDGGPVLADVIALVKDRANTL
REVAETALLFYRTEIAVAPELAAQHLTDDVRPGIVALADKLGALPEWKREAIGAAFKEVL
GAHGWKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDIVLRRLAA