Protein Info for RALBFv3_RS12605 in Ralstonia solanacearum IBSBF1503

Annotation: 16S rRNA pseudouridine(516) synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01479: S4
  • amino acids 13 to 49 (37 residues), 23 bits, 5.2e-09
• PF00849: PseudoU_synth_2
  • amino acids 69 to 200 (132 residues), 65.5 bits, E=6.5e-22
• TIGR00093: pseudouridine synthase
  • amino acids 73 to 231 (159 residues), 139.6 bits, E=3.9e-45

Best Hits

KEGG orthology group: K06183, ribosomal small subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 97% identity to rsc:RCFBP_20741)

Predicted SEED Role

"Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (252 amino acids)

>RALBFv3_RS12605 16S rRNA pseudouridine(516) synthase (Ralstonia solanacearum IBSBF1503)
MTASSARLPLYRILQSQGFGTRRYCKDLVIAGLVHVNGIEAEDPDAQVDTAGLVLDVDGE
RWAYHARAYLMLHKPAGFECSQKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLT
DDGQFVHTLTSPRHQVPKVYEITTADPVTDAQVAQLCRGVLLDDEDATVAAAWAERVDTC
HLRMALTQGKYHQVKRMVAAAGNHVTALHRSAIGALTLDAGLAPGQWRWLERDDLTALDT
TPVHAAGAATGA