Protein Info for RALBFv3_RS07045 in Ralstonia solanacearum IBSBF1503

Annotation: protein-disulfide reductase DsbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 612 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details transmembrane" amino acids 189 to 218 (30 residues), see Phobius details amino acids 231 to 253 (23 residues), see Phobius details amino acids 264 to 286 (23 residues), see Phobius details amino acids 309 to 338 (30 residues), see Phobius details amino acids 345 to 369 (25 residues), see Phobius details amino acids 377 to 399 (23 residues), see Phobius details amino acids 405 to 426 (22 residues), see Phobius details amino acids 439 to 460 (22 residues), see Phobius details PF11412: DsbD_N" amino acids 37 to 152 (116 residues), 111.8 bits, see alignment E=6.3e-36 PF13386: DsbD_2" amino acids 194 to 391 (198 residues), 41.4 bits, see alignment E=4.8e-14 PF02683: DsbD" amino acids 195 to 373 (179 residues), 75.5 bits, see alignment E=1.6e-24 PF13899: Thioredoxin_7" amino acids 497 to 577 (81 residues), 58.3 bits, see alignment E=2.2e-19 PF13098: Thioredoxin_2" amino acids 506 to 598 (93 residues), 41.5 bits, see alignment E=4.4e-14

Best Hits

Swiss-Prot: 91% identical to DSBD_RALSO: Thiol:disulfide interchange protein DsbD (dsbD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 97% identity to rsc:RCFBP_10460)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (612 amino acids)

>RALBFv3_RS07045 protein-disulfide reductase DsbD (Ralstonia solanacearum IBSBF1503)
MTLSAFLPLPLPLRRLLQLGMLLVAMATVVLPARAADDFLPPEQAFRFSAVQVDGQTVEV
KFAIADGYYMYRERLAVAADPATVTFAPLELPPGQVKFDETFNKDVETYRRALAFRVKVR
DAAGPFSLIVTSQGCADQGVCYPPMKSRFQVEPASASPAAPVNPPDAAESSDVLGGRIAS
TLGGGNLGAIAALFFGLGLLLTFTPCVLPMLPILSAIVVGEHATRMRATAVSLAYVLGMA
VVYTAVGVAAGLAGQGLQAALQNAWVLGAFAMLMVVLSLSMFGLYELQLPAAWRHHLTQA
SNRLSGGQVVGAAVMGALSALIVSPCVTPALAGALAYIAQTGNATVGGAALFSMAIGMGV
PLVLVGVGAGNLLPRAGYWLVVTKAVFGFILLGVALWIVQPVLPAWLAMVAWAVLLIAAA
VFLRTFDSLPADAGPLPRLGKVVGVVLAVAGAVQLVGMAAGGRDPLQPLAGVMRASTGAQ
ADARGVVFRRVKSVVEVDEAVRAASAAGRPVMLDFYADWCVSCKEMERLTFTDARVRAAL
ADVVLLQADVTADSADDRALLKRFALFGPPATIFFDAHGGQVSARVVGFERAETFLASLR
RAFGAVQAKPST