Protein Info for RALBFv3_RS06290 in Ralstonia solanacearum IBSBF1503

Annotation: phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 transmembrane" amino acids 22 to 45 (24 residues), see Phobius details amino acids 66 to 90 (25 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 134 to 152 (19 residues), see Phobius details amino acids 190 to 212 (23 residues), see Phobius details amino acids 222 to 246 (25 residues), see Phobius details amino acids 260 to 279 (20 residues), see Phobius details amino acids 286 to 307 (22 residues), see Phobius details amino acids 312 to 336 (25 residues), see Phobius details amino acids 367 to 386 (20 residues), see Phobius details PF10555: MraY_sig1" amino acids 68 to 78 (11 residues), 22.6 bits, see alignment (E = 5.4e-09) PF00953: Glycos_transf_4" amino acids 99 to 307 (209 residues), 111 bits, see alignment E=6.1e-36 TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 180 to 389 (210 residues), 283.2 bits, see alignment E=1.7e-88

Best Hits

Swiss-Prot: 99% identical to MRAY_RALSO: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 100% identity to rsc:RCFBP_10613)

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (389 amino acids)

>RALBFv3_RS06290 phospho-N-acetylmuramoyl-pentapeptide- transferase (Ralstonia solanacearum IBSBF1503)
MLLALAQWLQNDFGFLRVFNYLTFRAVMASLTALVIGLGFGPWVIRRLTELKVGQAVRSY
GPQTHLVKAGTPTMGGVLVLIGIAVSTLLWCDWGNRFIWIVLLVTLGYGAIGWVDDYRKV
VHRDPKGMSSREKFFWQTVIGLFAAAYLAFSVSETSNMRVLDLFLDWVRSGLSLSLPAKS
HLIVPFFKEISYPLGVFGFIVLTYLVIVGSSNAVNLTDGLDGLVIMPVVLVGSALGIFAY
VMGSAVYSKYLLFPHIPGAGELLIFCSAMAGAGLAFLWFNAHPAQVFMGDVGALALGGAL
GTIAVIVRQEIVLFIMGGVFVAETISVMLQVTWFKFTKRRYGEGRRLFRMAPLHHHFELS
GWKETQVVVRFWVITMMLVLIGLSTLKLR