Protein Info for RALBFv3_RS06275 in Ralstonia solanacearum IBSBF1503

Annotation: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 transmembrane" amino acids 9 to 30 (22 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 99 to 124 (26 residues), see Phobius details TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 5 to 354 (350 residues), 339.2 bits, see alignment E=1.3e-105 PF03033: Glyco_transf_28" amino acids 10 to 146 (137 residues), 119.5 bits, see alignment E=1.2e-38 PF04101: Glyco_tran_28_C" amino acids 186 to 350 (165 residues), 128.4 bits, see alignment E=2.9e-41

Best Hits

Swiss-Prot: 95% identical to MURG_RALSO: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 97% identity to rsc:RCFBP_10616)

MetaCyc: 50% identical to N-acetylglucosaminyl transferase (Escherichia coli K-12 substr. MG1655)
acetylglucosaminyltransferase. [EC: 2.4.1.227]

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (361 amino acids)

>RALBFv3_RS06275 undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (Ralstonia solanacearum IBSBF1503)
MTAGTPRTLLVMAGGTGGHIFPALSVARLLAARGWKVVWLGNAGSMEGQLVPKHGFPLES
VRFGGLRGKGLLTKFLLPLNLLRAFWQSLGVLRRVRPNVVLGMGGYITFPGGMMSVLLGA
PLVLHEQNSIAGLANRVLARVADRVLCAFPNALPGAEWVGNPIRADLAALPSPQARYAER
SGPLRVLVVGGSLGAAALNDAVPKALALLPADTRPIVVHQAGAKQIETLRANYAAAGIDE
THAQAVPFIDDMAAAYAQADLVICRAGAMTVSEVAAAGVAALFVPFPHAVDDHQTTNARF
LSERGAALLVPQPSLGPASLADTLASLTRAQLADMAAKAREQARPEAAERVADICAAAAR
A