Protein Info for RALBFv3_RS03750 in Ralstonia solanacearum IBSBF1503

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 944 transmembrane" amino acids 769 to 787 (19 residues), see Phobius details PF02739: 5_3_exonuc_N" amino acids 7 to 169 (163 residues), 195.5 bits, see alignment E=1.1e-61 TIGR00593: DNA polymerase I" amino acids 8 to 944 (937 residues), 917.2 bits, see alignment E=6.9e-280 PF01367: 5_3_exonuc" amino acids 170 to 254 (85 residues), 89.9 bits, see alignment E=2.5e-29 PF01612: DNA_pol_A_exo1" amino acids 334 to 524 (191 residues), 121.1 bits, see alignment E=9.3e-39 PF00476: DNA_pol_A" amino acids 558 to 942 (385 residues), 530.8 bits, see alignment E=4e-163

Best Hits

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (944 amino acids)

>RALBFv3_RS03750 DNA polymerase I (Ralstonia solanacearum IBSBF1503)
MSESQETLLLVDGSSYLYRAYHALPDLRNGEGFPTGAIYGIVNMLRKLRNDYPAKYSACV
FDAKGKTFRDELYPAYKEHRAPMPDDLRQQIEPIHEAVRALGWPILVVDGVEADDVIGTL
AERAAREGIRTVVSTGDKDLAQLVNDHVTLVNTMSNETLDPAGVQAKFGVPPEKIVDYLS
LIGDTVDNVPGVPKVGPKTAVKWLSEYGSLDNVIARAGEIKGVVGENLRNTLDWLPKGRE
LLTVKTDCDLSPDVPSFDALVDGGEDRARLADFFGRYGFKTWLREATGESLPDTRSVGAA
RKAATPVKQYAPEAAAQQQASLFDVETPPAEVKYETVTTEAQLESWMRLLDASELVCIDT
ETTSIDPMLAQLVGISLSVQPGQACYIPVAHRGPDVSGLDTAAQLSREAVLARMRLWLED
ETRGKVGQHLKYDAHVFANHGIALRGITHDTMLQSYVLASHRNHGMDALAERVLHLKTIT
YEEVCGKGAAQIGFDEVALDRATEYAAEDADITLRLHRALYPKVAEDDKLRYVYERIEMP
TSIVLQKMERNGVLVDAERLARQSAELGQRLLALEQEAYALAGQPFNLNSPKQIGEIFFT
QLKLPVVKKTASGAPSTDEEVLQKLAEDYPLPKLLLDYRGLAKLKSTYTDKLPKMVNPQT
GRVHTTYGQATAVTGRLASTDPNLQNIPVRTEEGRRIREAFVAPEGSVIVSADYSQIELR
IMAHLSGDDGLMRAFREGQDVHRATAAEVFGVTPLEVTAEQRRYAKVINFGLIYGMSAFG
LASNLGIGRDAAKLYIDRYFARYPGVARYMDETRQIAHERGYVETVFGRRLWLPDINGGN
GPRRQAAERAAINAPMQGTAADLIKLSMLAVQDWLEGSPNADKPGTPMRSRLVMQVHDEL
VLEVPQDELAQVRERLPELMCGVASLSVPLVAEVGVGSNWEEAH