Protein Info for RALBFv3_RS00360 in Ralstonia solanacearum IBSBF1503

Annotation: 16S rRNA pseudouridylate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 PF01479: S4" amino acids 425 to 471 (47 residues), 33.6 bits, see alignment 2.6e-12 PF00849: PseudoU_synth_2" amino acids 487 to 642 (156 residues), 54.6 bits, see alignment E=1.5e-18 TIGR00093: pseudouridine synthase" amino acids 524 to 671 (148 residues), 114.1 bits, see alignment E=2.7e-37

Best Hits

KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 98% identity to rsc:RCFBP_20032)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (677 amino acids)

>RALBFv3_RS00360 16S rRNA pseudouridylate synthase (Ralstonia solanacearum IBSBF1503)
MTDSDDLPGDDTKRLANDPTQRATRPGKRATLGVRRDDAGNPVRTTGKPLRASDLNRDRQ
PLRPSQRRPESQEGAARKPPRPRRDDDGQPPREAKPRRMQGDRPPRRFDDERPPRRFGDD
QRPPRRFDDERPPRRFEDRPPRRSDDERPPRRFGDDQRPPRRFGDERPPRRFEDRPPRRS
DDERPPRRFGDDQRPPRRFDDERPPRRFEDRPPRRSDDERPPRRFGDDQRPPRRFGDERP
PRRFEDRPPRRSDDERPPRRFGDDQRPPRRFGDERPPRRFEDRPPRRSDDERPPRRFGDD
QRPPRRFDDERPPRRFEDRPPRRFDDERPPRKFGDDQRPPRRSDDERPPRRFEDRPPRRS
DDERPPRRYGDDRAPRRFDDEQRPPRRFEDRPARAPRPEREERAPRREEEHTPRPAAQAE
SSGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVRPDAEIEILPAARSE
QGERVTVLLHKPMGYVSGQAEDGYEPAATLFTAENQWEEDPTHKRFAAWQHKGLAPAGRL
DIDSTGLLVLTQDGRVARHLIGEDSTVEKEYLVRVVWDAPQGPVEHDISKVFPEELLEKL
RHGLSLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQVGLTVVGLKRIRMGSVVL
GDLPVGKWRFLGRFEKF