Protein Info for Psyr_4293 in Pseudomonas syringae pv. syringae B728a

Annotation: Acyl-CoA thioesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00189: acyl-CoA thioesterase II
  • amino acids 12 to 283 (272 residues), 311.3 bits, E=3.3e-97
• PF02551: Acyl_CoA_thio
  • amino acids 25 to 104 (80 residues), 22.1 bits, E=1.8e-08
  • amino acids 173 to 281 (109 residues), 109.1 bits, E=2.2e-35
• PF13622: 4HBT_3
  • amino acids 34 to 109 (76 residues), 81.1 bits, E=8.6e-27
• PF20789: 4HBT_3C
  • amino acids 156 to 282 (127 residues), 87 bits, E=2e-28

Best Hits

Swiss-Prot: 52% identical to TESB_ECOLI: Acyl-CoA thioesterase 2 (tesB) from Escherichia coli (strain K12)

KEGG orthology group: K10805, acyl-CoA thioesterase II [EC: 3.1.2.-] (inferred from 100% identity to psb:Psyr_4293)

MetaCyc: 52% identical to acyl-CoA thioesterase II (Escherichia coli K-12 substr. MG1655)
Acyl-CoA hydrolase. [EC: 3.1.2.20]; 3.1.2.- [EC: 3.1.2.20]; 3.1.2.- [EC: 3.1.2.20]; 3.1.2.- [EC: 3.1.2.20]

Predicted SEED Role

"Acyl-CoA thioesterase II (EC 3.1.2.-)" (EC 3.1.2.-)

MetaCyc Pathways

• oleate β-oxidation (30/35 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (2/2 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• acyl-CoA hydrolysis (1/1 steps found)
• palmitoleate biosynthesis IV (fungi and animals) (1/2 steps found)
• firefly bioluminescence (2/14 steps found)
• jasmonic acid biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis
• Limonene and pinene degradation
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.-

Use Curated BLAST to search for 3.1.2.- or 3.1.2.20

Sequence Analysis Tools

See Q4ZNE9 at UniProt or InterPro

Protein Sequence (289 amino acids)

>Psyr_4293 Acyl-CoA thioesterase (Pseudomonas syringae pv. syringae B728a)
MSQVLDDLVELLTLEAIEENLFRGHSQDLGFRQLFGGQVLGQSLSAASQTVEEDRHVHSL
HGYFLRPGDAGLPVVYQVDRVRDGGSFSTRRVTAIQKGKPIFTCSASFQYDEEGFEHQAT
MPQIVGPEDLPSELELLTRRAHLIPEAMHDKFLRSKPIQFRPVTAEDPYDPKPGDPVKYV
WFRADGSLPDVQALHRYMLAYASDFNLLTTSLLPHGKTVWQRDMQVASLDHSLWFHNDLR
ADDWLLYAMDSPWAGNSRGFSRGSIFNRTGQLVASVSQEGLIRHRKDWA