Protein Info for Psyr_4194 in Pseudomonas syringae pv. syringae B728a

Annotation: DnaJ central region:Heat shock protein DnaJ, N-terminal:Chaperone DnaJ, C-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 380 PF00226: DnaJ" amino acids 5 to 67 (63 residues), 91.4 bits, see alignment E=4.5e-30 TIGR02349: chaperone protein DnaJ" amino acids 5 to 354 (350 residues), 459.7 bits, see alignment E=4.1e-142 PF01556: DnaJ_C" amino acids 125 to 338 (214 residues), 181.4 bits, see alignment E=1.7e-57 PF00684: DnaJ_CXXCXGXG" amino acids 152 to 212 (61 residues), 63.3 bits, see alignment E=3e-21

Best Hits

Swiss-Prot: 100% identical to DNAJ_PSEU2: Chaperone protein DnaJ (dnaJ) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K03686, molecular chaperone DnaJ (inferred from 99% identity to pst:PSPTO_4504)

MetaCyc: 64% identical to chaperone protein DnaJ (Escherichia coli K-12 substr. MG1655)
1.8.4.-

Predicted SEED Role

"Chaperone protein DnaJ" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNP8 at UniProt or InterPro

Protein Sequence (380 amino acids)

>Psyr_4194 DnaJ central region:Heat shock protein DnaJ, N-terminal:Chaperone DnaJ, C-terminal (Pseudomonas syringae pv. syringae B728a)
MAKRDYYEVLGVERGSSEADLKKAYRRLAMKHHPDRNPDDKASEELFKEANEAYEVLSDA
SKRAAYDQYGHAGVDPSMGGGGGFGGGAGGANFSDIFGDVFSDFFGGGRAGGGGGRGGAQ
RGSDLRYTLELNLEEAVRGTNVNIRVPTLVNCKPCDGSGAKKGSSPVTCPTCGGIGQVRM
QQGFFSVQQTCPRCHGHGKIISDPCDSCHGEGRVEESKTLSVKVPPGVDTGDRIRLSGEG
EAGTQGGPTGDLYVVINVREHAIFQRDGKHLFCEVPISFTDAALGGELEVPTLDGRVKLK
IPEGTQTGKQFRLRGKGVAPVRGGGAGDLMCRVAVETPVNLSKRQRELLEEFRTSLENDE
SHSPKASGWFEGVKRFFGDL