Protein Info for Psyr_3886 in Pseudomonas syringae pv. syringae B728a

Annotation: methionyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 682 PF09334: tRNA-synt_1g" amino acids 7 to 395 (389 residues), 516.2 bits, see alignment E=1.3e-158 TIGR00398: methionine--tRNA ligase" amino acids 7 to 534 (528 residues), 579.2 bits, see alignment E=8.5e-178 PF00133: tRNA-synt_1" amino acids 11 to 75 (65 residues), 31.8 bits, see alignment E=1.7e-11 PF19303: Anticodon_3" amino acids 418 to 517 (100 residues), 32.1 bits, see alignment E=2.5e-11 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 559 to 682 (124 residues), 139.3 bits, see alignment E=7.2e-45 PF01588: tRNA_bind" amino acids 586 to 679 (94 residues), 87.2 bits, see alignment E=1.6e-28

Best Hits

Swiss-Prot: 100% identical to SYM_PSEU2: Methionine--tRNA ligase (metG) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to psb:Psyr_3886)

MetaCyc: 64% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.10

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPK6 at UniProt or InterPro

Protein Sequence (682 amino acids)

>Psyr_3886 methionyl-tRNA synthetase (Pseudomonas syringae pv. syringae B728a)
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIML
RAEKEGITPEQLIANVKAEHSADFADFLVEFDNFHSTHSDENRELSSMIYKRLRDAGHIA
TRSVTQYFDPEKKMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTDLKDPKSAISGA
TPVLKDSKHFFFDLPAFDAMLKSWTRSGTLQDAVANKIAEWLDSGLQQWDISRDAPYFGF
EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPDLDFDAYWGKGATTELYHFIGKDIVNFH
ALFWPAMLEGAELRTPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLPPEYLRYYYAS
KLGRGVDDLDLNLEDFVQKVNSDLIGKVVNIASRCAGFIHKGNAGVMVEANAAPELTDAF
LTAAPSIADAYEARDFARAMRETMALADRANAYIAEKAPWALAKQEGRQDEVQAVCALGI
NLFRQLVIFLKPVLPNLAADAEKFLNVEPLTWEDHKTLLANHQLNPFSALMTRIDPVKVE
AMATASKEDLTATDSSADTAPAGNGELAKDPLSAEIDFDAFAAIDLRVALILKAEHVEGA
DKLLRLTLDIGDEQRNVFSGIKSAYPNPSELEGRLTMMIANLKPRKMRFGISQGMVMAAG
PGGEEIYLLSPDSGARPGQRIK