Protein Info for Psyr_3804 in Pseudomonas syringae pv. syringae B728a

Annotation: ATP-dependent DNA helicase, RecQ-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 17 to 344 (328 residues), 240.8 bits, see alignment E=1.4e-75 PF04851: ResIII" amino acids 20 to 187 (168 residues), 45.4 bits, see alignment E=1.7e-15 PF00270: DEAD" amino acids 23 to 194 (172 residues), 88.5 bits, see alignment E=8.8e-29 PF00271: Helicase_C" amino acids 240 to 340 (101 residues), 50 bits, see alignment E=6.4e-17 PF00156: Pribosyltran" amino acids 573 to 686 (114 residues), 29.3 bits, see alignment E=1e-10

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 100% identity to psb:Psyr_3804)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPT7 at UniProt or InterPro

Protein Sequence (698 amino acids)

>Psyr_3804 ATP-dependent DNA helicase, RecQ-like protein (Pseudomonas syringae pv. syringae B728a)
MQRNDAEKLLKVAINNGAATFRDGQWEAIDALVNHRKKLLVVQRTGWGKSSVYFISTRIL
RDRGQGPTIIISPLLALMRNQVDAAQRLGINAVTINSTNTDEWEKARQDVHANRVDCLLI
SPERLANDGFMETFLQPIADRIGLMVIDEAHCISDWGHDFRPDYRRIVNILRFLPPNTPV
LGTTATANTRVVNDIQAQFGDINIVRGPLARESLALQNLIMPDQASRLAWLAQTIPTLPG
TGIVYALTQRDSEQVARWLRANGIDAASYHSDVAHSDFGNSDQYRQHLEDELLANKLKVL
VATTALGMGYDKPDLGFVIHYQAPSSLVAYYQQVGRAGRAIDYSLGLLMSGEEDEKIHAF
FRRAAFPPAGQVNHLLSVLAKSNGLTIRELEEHSNLSHGQIEKILKLLSVESPAPLIKHQ
SKWLRTPIHYELDQNRVSRLTELREHEWQQVLAYLNEPRCLMAFLRHALDDAENDACGKC
VRCLERELVSSKVDGRLVHQAATFIRHAELPIKPKKQIAKGGFEYYGFPTNLPLNIQAAE
GRVLSQWRDGSWGELAANGKELGHFDDALVSAMAEMIQSRWQPNPKPTWLCCVPSLGHPM
LVPDFAQRLANALGIPFVNCVTKVRQNEPQKWQNNRFHQCRNLDGVFAVSAHIPPGPVLL
VDDMIDSGWTMTVIAALLKQAGSEHVYPLAISSTASNA