Protein Info for Psyr_3800 in Pseudomonas syringae pv. syringae B728a

Annotation: Major facilitator superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 420 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 59 to 79 (21 residues), see Phobius details amino acids 88 to 111 (24 residues), see Phobius details amino acids 117 to 136 (20 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 177 to 198 (22 residues), see Phobius details amino acids 219 to 240 (22 residues), see Phobius details amino acids 252 to 273 (22 residues), see Phobius details amino acids 281 to 302 (22 residues), see Phobius details amino acids 309 to 331 (23 residues), see Phobius details amino acids 343 to 364 (22 residues), see Phobius details amino acids 370 to 391 (22 residues), see Phobius details PF07690: MFS_1" amino acids 26 to 338 (313 residues), 129.5 bits, see alignment E=7.2e-42

Best Hits

Swiss-Prot: 53% identical to OPDE_PSEAE: Transcription regulatory protein OpdE (opdE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03445, MFS transporter, DHA1 family, purine ribonucleoside efflux pump (inferred from 100% identity to psb:Psyr_3800)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPU1 at UniProt or InterPro

Protein Sequence (420 amino acids)

>Psyr_3800 Major facilitator superfamily (Pseudomonas syringae pv. syringae B728a)
MQAFDSTLKQSEITTHPPAWGAVFSMALCVVVLIASEFMPVSLLTPIAQDLGISQGQAGQ
AISISGFFAVLTSLLNTLLTGHLDRKPVLLGFSLLLLVSGVIVTLASNGWLFMTGRALLG
IAIGGFWSMSTATVMKLVPKQSVAKGLALINGGNALAATVAAPLGSFMGQYIGWRGAFFL
VIPLAALAFAWQWLSLPAMRSQPNNRARNPFRLLRNPQVAVGMSAILLLFMGQFAVFTYL
RPFLEEITGVSINTLSLMLLALGASGLVGTYLIGRLLHKGLYACLIAIPLLMAVLAVALT
GLGHSPQSVAIVLAVWGLIATPAPVAWGLWLSRTLPDDAEAGGGLMVATIQLAITAGAGI
GGALFDSLGWWSPFAFGGVLLACSAALAWTARDNALLQEKNTGQGRADKRGSISQRGNTP