Protein Info for Psyr_3733 in Pseudomonas syringae pv. syringae B728a

Annotation: Outer membrane autotransporter barrel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1038 signal peptide" amino acids 1 to 43 (43 residues), see Phobius details PF00082: Peptidase_S8" amino acids 83 to 420 (338 residues), 149.3 bits, see alignment E=2.3e-47 TIGR02601: autotransporter-associated beta strand repeat" amino acids 495 to 526 (32 residues), 44.4 bits, see alignment (E = 1.1e-15) PF12951: PATR" amino acids 496 to 525 (30 residues), 44.7 bits, see alignment (E = 1.6e-15) TIGR01414: outer membrane autotransporter barrel domain" amino acids 560 to 1038 (479 residues), 155.4 bits, see alignment E=2.5e-49 PF03797: Autotransporter" amino acids 766 to 1018 (253 residues), 220.8 bits, see alignment E=4.1e-69

Best Hits

KEGG orthology group: K12685, subtilase-type serine protease [EC: 3.4.21.-] (inferred from 100% identity to psb:Psyr_3733)

Predicted SEED Role

"Outer membrane stress sensor protease DegS" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQ08 at UniProt or InterPro

Protein Sequence (1038 amino acids)

>Psyr_3733 Outer membrane autotransporter barrel (Pseudomonas syringae pv. syringae B728a)
MKDAFSTRHTSVKSLRQAIRAVNRASCGALACYLATLGVAHAAPYVEAGQAGNAASWRSA
EFNADWGLGAINADQAYAAGYSGKDIKLGIFDQPVYAPHPEFDSPNKVVNLVTSGIREYT
DPYIPVKAGDAFRYDGAPSLDSGGKLGNHGTHVGGIAGGNRDGGPMHGVAYNAQIISADN
GDPGPEDGIVLGNDGAVYQAGWNALVNSGARVINNSWGIGITDRFDKGGRDPAFPHFTVQ
DAQVQFDQIRQILGTRPGGAYQGAIDAARSGVVTIFAAGNDYNLNNPDAMAGLGYFVPDI
APNWLTVAALQQNPDAAAAATTPYTLSTFSSRCGYTASFCVSAPGTRIYSSVLNGTSLAD
LTVGWANKNGTSMAAPHVAGSMAVLMERFPYMTGAQVADVLKTTATDLGAPGVDALYGWG
MINLGKAINGPSMFVTEADIPAEFRIDGAYGDSQFVADLPGVGAIVDAGKPTQRTCSGPQ
CGLDVWSNDISGHGGLTKQGIGTLVLSGANSYSGPTLVNQGRLAINGSLASAVTVNNGGI
LGGNGSVASLNANQGGTVAPGNSIGTLQVAGDLNLAPGSTYAVEFSPTASDRIVVGGTAT
VSGANMALSLENDNPALLSSNQTSSVLGQQFNVLQAAGGVQGQFGSVTDNYAFLDGNLAY
TGTGVTLALARNGDSFASAAQTDNQRSVAQAIEQLSAGNSVYESIILSQDTASARRAFTQ
LSGEVHPAIATQLINDSRQLRDAVGDRLRVEGLYDQPAPGSEDNSVWVKALGAWGKNSGS
SDSASSTSSLGGLLAGVDGLISEHTRLGAMAGYSDTSLSLGDDTHSRASADSYHLGAYIG
HEQGALRLTAGASHSWHRIDVKRDLQFGAFSDRQKVKRDAQSSQVFTEAAYRLNLQPLAL
EPFANLAYVHFDSDSFTEKGGATALKGSDDTRDTVLSTLGMRAGNRFNLNDTQKLDVSAT
LGWQHNLSDTSSEQHLAFASAGNSFNVQSVSMDRDAAVVGARASLALGRDARINLDYNGL
LGARDKTHGVGLSLDWQF