Protein Info for Psyr_3714 in Pseudomonas syringae pv. syringae B728a

Annotation: Ferric iron reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR03951: siderophore-iron reductase FhuF" amino acids 69 to 247 (179 residues), 194.2 bits, see alignment E=1.1e-61 PF06276: FhuF" amino acids 73 to 218 (146 residues), 85.1 bits, see alignment E=7.9e-28 PF11575: FhuF_C" amino acids 226 to 246 (21 residues), 28.6 bits, see alignment (E = 1e-10)

Best Hits

KEGG orthology group: K13255, ferric iron reductase protein FhuF (inferred from 100% identity to psb:Psyr_3714)

Predicted SEED Role

"Ferric reductase (1.6.99.14)" in subsystem Anaerobic respiratory reductases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQ26 at UniProt or InterPro

Protein Sequence (248 amino acids)

>Psyr_3714 Ferric iron reductase (Pseudomonas syringae pv. syringae B728a)
MLQPDLAAHAGAALSLPLFAGPFARFGRTLLTADDPRPVMTLPDLLRPERLDQILLTVYG
PQLMPDQLPVLVSQWAKFYFMQLIPPVLVASLVHDWHWPLQLEQVALALDERGVPSGIRL
AGEGSVWRGIAVDPFQRFAGLLDDNLQPFITSLSAYGGLSAAVLWSSAGDYLEGCLAQLA
TCSDASLAAGLALLSEKKRPDGRTNPLFQTVRYVPQARGGEPRRQRRVCCLSHRVEWVGR
CEHCPLPG