Protein Info for Psyr_3225 in Pseudomonas syringae pv. syringae B728a

Annotation: Protein-tyrosine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 735 transmembrane" amino acids 32 to 55 (24 residues), see Phobius details amino acids 445 to 464 (20 residues), see Phobius details PF02706: Wzz" amino acids 16 to 106 (91 residues), 60 bits, see alignment E=8.2e-20 PF23607: WZC_N" amino acids 113 to 223 (111 residues), 104.5 bits, see alignment E=1.5e-33 PF13807: GNVR" amino acids 386 to 467 (82 residues), 98.8 bits, see alignment E=4.6e-32 TIGR01007: capsular exopolysaccharide family" amino acids 522 to 721 (200 residues), 156.9 bits, see alignment E=2.6e-50 PF10609: ParA" amino acids 540 to 659 (120 residues), 31.7 bits, see alignment E=3.9e-11 PF01656: CbiA" amino acids 544 to 715 (172 residues), 34.4 bits, see alignment E=7e-12 PF13614: AAA_31" amino acids 545 to 664 (120 residues), 41.1 bits, see alignment E=6.4e-14 PF09140: MipZ" amino acids 548 to 658 (111 residues), 23.1 bits, see alignment E=1.5e-08

Best Hits

KEGG orthology group: K00903, protein-tyrosine kinase [EC: 2.7.10.-] (inferred from 100% identity to psb:Psyr_3225)

Predicted SEED Role

"Tyrosine-protein kinase Wzc (EC 2.7.10.2)" in subsystem Colanic acid biosynthesis (EC 2.7.10.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.10.- or 2.7.10.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRG5 at UniProt or InterPro

Protein Sequence (735 amino acids)

>Psyr_3225 Protein-tyrosine kinase (Pseudomonas syringae pv. syringae B728a)
MTAMNHPSLNFQPDSRIDLATVLRMLFDHKALILWTVGVFFLIGLGYAILATPVYQATAM
IQIEPRKIGIEGTPEVSAKPLSVSQATTEIELIKSRALLGKVVDDLQLNRLQTPDLFPVI
GPYLYRTFKPTRDGELAQPLFGLTQYAWGGEKIEVFQLEVPEHLLGEKLTLTAGKPGQFS
LYDSEHTLLLGGAINRVVEGHGIKIQVATLQARPGTDFTVSRQRTLSTALIYQNRLKIAE
AGRDSGIIYLSIEDQDAQRANRILDEVSHLYVRQNVERSSAEAAQRLQFLRSQLPAVRKQ
LEESETALNTFQTSARSVDLSIETKGVLDQVVSLDSMLSELKLKRVELERLYTREHPTYR
SLMSQINQLEQQRQGLLKKIDALPMTQQELLRLTRDMQVISQTYTLMLNKSQEQDILRAG
NIGNVRVIDNADTNVERPVKPMKTLIVLIATLLGALVAMTMVFVRQAFYRGVESAEIIEN
LGMPVYASLPHSRQQEHLDKRDHAGKESKLLSIAAPAELAIESLRSLRTSLHFAMLEARN
NVLMISSPTPGAGKSFVSSNLATIIAQTGKRVLLIDADMRKGYLHRLFGLQPKHGLSDTL
AARLRCTEVINTTRVRHLDFISCGFAAPNPSELLMHDNFHKMLAELSPLYDLILIDTPPI
LAVTDATLVGRQAGTCLLVARFGMTTVQEIEASKRRLGQNGILIKGAIFNAVVRKATTSD
YDCAAYGYNYHPAPR