Protein Info for Psyr_2597 in Pseudomonas syringae pv. syringae B728a

Annotation: diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 58 to 79 (22 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 101 to 260 (160 residues), 103.9 bits, see alignment E=3.6e-34 PF00990: GGDEF" amino acids 104 to 257 (154 residues), 117.3 bits, see alignment E=5.8e-38 PF00563: EAL" amino acids 278 to 507 (230 residues), 221.8 bits, see alignment E=8.7e-70

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2597)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZT86 at UniProt or InterPro

Protein Sequence (534 amino acids)

>Psyr_2597 diguanylate cyclase/phosphodiesterase (Pseudomonas syringae pv. syringae B728a)
MTFLRSPHMVRKYLYTVLWFYVLSIFLLAIAWEFELESLAMAAMGLPYDQDFENAERWRF
VLTSTGFAVLSMVIPFILLKRLMQQLHSSYNDLLESQALSESLARHDPLSGLLNRRVFHE
QLVARLQQPATQTAVFLIDLDKFKLINDTHGHAAGDAAICAVAESLRDATVGWQASVARL
GGDEFGLAVSGDFSHAELAPLAENILAKIAASSVGLPRVALSATLGIAIAPMDGSDPDLL
LQRADSAMYRGKAGGRARFHFYEVSFEREQRLQEQFARELTLAIEEKDIQPFYQPIVSLP
EQRLAGFELLARWLHPERGIILPLDFIPVAEQLGLIKPLTATLLLQAFDQARDWPSDFTL
AINVTSLMIESPDFPDWLEQLANEGKFPLSRLEVEVTENALVANVDSARLNLERLRAMGV
SVALDDFGTGYSGLYHLTRLAIDKIKIDRSFFDTALENQNEMVKAILALGKSLRMKITAE
GVEHERLADWLATHECDFAQGYLFGRPLPLAQVKELLAMSGSEGPVRALAVPGK