Protein Info for Psyr_2432 in Pseudomonas syringae pv. syringae B728a

Annotation: diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 969 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF13185: GAF_2" amino acids 164 to 310 (147 residues), 29.1 bits, see alignment E=2.1e-10 amino acids 365 to 505 (141 residues), 39.2 bits, see alignment E=1.6e-13 PF01590: GAF" amino acids 165 to 309 (145 residues), 26.7 bits, see alignment E=1.4e-09 amino acids 387 to 505 (119 residues), 33.2 bits, see alignment E=1.4e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 539 to 703 (165 residues), 138.2 bits, see alignment E=1.1e-44 PF00990: GGDEF" amino acids 544 to 699 (156 residues), 165.3 bits, see alignment E=2e-52 PF00563: EAL" amino acids 721 to 954 (234 residues), 230.1 bits, see alignment E=5e-72

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2432)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZTQ1 at UniProt or InterPro

Protein Sequence (969 amino acids)

>Psyr_2432 diguanylate cyclase/phosphodiesterase (Pseudomonas syringae pv. syringae B728a)
MHLDASMTVSLLATGLCPSVACTSIAPGSALPATRDRFSCHASIEALISAARQQTGAIAW
AAYAAGELKCLLGQGDESVVWPTHIAAEDFEAYCTQLQLHCRVAGQGEGVLGWVLAPMAE
KDHPLLLDLAVRLGSLLQTAALIRAQNTQRVLYEISLLASTTLDRGAFLQGIHEQLGTLI
DAENFYVALYDRQSGKITYPYYIDRFDDEAMAHETFEFIDADRMSLTGYVLSSGQPLFLD
AACIEQAKAENRFYCVGRLPEFWMGAPLKNASDDVFGMVAMQVYDVSRVYSAQDRALFAV
VSRHVAMALDRQLHRTDLEQVVALRTQQLSAANDALRQEVRDRERAEHLQNALFQIAELS
SQPGDMNELFRSLHMIVGELLVALNFYIALYDSATGEVTFPYYIDEFKPAPPRSRRGQRG
FTEYVIGQRRPCLIDYEEARRLEGLGEIEVQRQSGRCSSWLGIPLFDGGQVRGVLVVQSY
SKDVSYTLRDQELLTFVSRHIDTALSRRSAAEAIHTANVLLEARVRDRTRELDRANARLQ
HENSHDSLTGLPNRSHLQYRLRQAWQDFCRNGQQVIVMFIDLDRFKMINDSLGHHCGDIL
LVQAAERLRSCMRERDLLARLGGDEFAVLSIDTPLQTGTEIAQRILTAFEQPFVIDDHTV
FSSCSIGVVGADIRFHQEPADLLRDADTAMYRVKNGGRDSFAVFNQALRREVSDQVEQEG
ALRTALKRTDQLIPYFQPVICVNSGKLLAFEALIRWCMPDGRIVGPESFLPALEGLRLIG
RLDLYMLRRVVSILAQPENSHWPPVHVNCSSYSITRPAFAAEVLALLAEQNVAPERLCLE
LTEGALVADPEQARLSMKLLAEHGMSVMLDDFGAGFSSLSYVHQYHFSGLKIDKSFIFGL
TSSVRSRAIVRAIVRMAESLDLTVVAEGVEDRETLELLREIGATQAQGYYFSEPLPIELL
DINTGLAAG