Protein Info for Psyr_2385 in Pseudomonas syringae pv. syringae B728a

Annotation: Phytochrome:GAF:ATP-binding region, ATPase-like:Histidine kinase A, N-terminal:HWE histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1003 PF08446: PAS_2" amino acids 26 to 129 (104 residues), 84.1 bits, see alignment E=4.4e-27 PF01590: GAF" amino acids 154 to 315 (162 residues), 60.2 bits, see alignment E=1.3e-19 PF00360: PHY" amino acids 338 to 527 (190 residues), 130.5 bits, see alignment E=1.9e-41 PF00512: HisKA" amino acids 542 to 612 (71 residues), 52.2 bits, see alignment 2e-17 PF02518: HATPase_c" amino acids 658 to 765 (108 residues), 100 bits, see alignment E=4.3e-32 PF07568: HisKA_2" amino acids 811 to 873 (63 residues), 24.8 bits, see alignment 7.6e-09 PF07536: HWE_HK" amino acids 811 to 890 (80 residues), 81.8 bits, see alignment E=1.9e-26

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2385)

Predicted SEED Role

"Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)" in subsystem Oxidative stress or Oxygen and light sensor PpaA-PpsR (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZTU8 at UniProt or InterPro

Protein Sequence (1003 amino acids)

>Psyr_2385 Phytochrome:GAF:ATP-binding region, ATPase-like:Histidine kinase A, N-terminal:HWE histidine kinase (Pseudomonas syringae pv. syringae B728a)
MEFTQLIKDMLDAKPDAALEAALAECAREPIRVPGAIQPHGVLLSVAGDPLCIEQVSANC
ATEFGMAADELLGKPLSWLLSPEQSALIDHAYGHPAAPHIDPIKLTIGTAHYSASLQRAD
DVLIIELEPFVETGHGQSRIITRVLRNLQAATTLETLFDISVHEIQALTGYDRVMIYRFE
PEGHGQVVAQALTGPLPSYSGLNFPGSDIPAQARELYRLNWIRVIPDATYVPVPLIPTLR
PATGQPLDLGFSTLRSVSPVHCEYLKNMGVRSSMSISLLDGGELWGLVACGHPEPLRVSR
ELRDACAMIGQLLSVKISAIVATNIQREREEKVVLLGQLADAMSRANHEVLDGLVSRPEL
LMSLTLADGAAVLIDDQVYLFGTCPSIEQVRELYTWIRDTGLASQRSRQRATGLQGLGVF
HTNSMQRELADSAVYRDIASGVIAFTLPKPIDNAVMWFRAQLTSTMNWSGNPTHHVSTRP
ESSASHRLHPRQSFDVWEQEVTGIASPWSRADLYGAEDIRRSALESDLERQVQREQEAVR
LRDELVAVVSHDLRNPMSIIIMQCGMMQSWAVGDTHFENRNIRRALGTIEKATTRMNSLL
EDLLDTAQIEAGRYQLSRLPLSVTSLLEEACSLLVMLTTEKNIELNCTAAQGLVIDADPE
RIFQVLSNLVGNAIKFTPKGGRINIDAVADQNEVLFRVSDDGIGIPAEHLPFIFQRYWSV
KEGNPRGNGLGLYICQGIITAHGGRLWADSSLDSGSVFSFTLPVHQGTDSIGETTFLKQS
GTTHRLAQSISSKLERQQLEDRLTRAGLLNELNHRVKNTLATVQAIASLTVNSSASLESF
HKSFGARLFALSQAHDALARAEWISTELVDLIEQLQEQDSGAHRISFEGDPVTLEPRFSL
TLSMVLHELMANALQHGALSSASGQVTVTSTLTAEHTPPTLKIEWKETGGPPVVATTVKG
FGLRLIRRSIERELKGQADIQFARTGIIWSMLIPWPDKPESRL